Increased telomerase activity and hTERT expression in human salivary gland carcinomas
Increased telomerase activity and hTERT expression in human salivary gland carcinomas
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DOI：
10.3892/ol.2011.354
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发表时间：
2011-09-01
期刊：
影响因子：
2.9
通讯作者：
Kamata, Nobuyuki
中科院分区：
文献类型：
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作者：
Shigeishi, Hideo;Sugiyama, Masaru;Kamata, Nobuyuki
大约85%的人类恶性肿瘤表达端粒酶水平升高。端粒酶活性与恶性组织的显著关联提供了强有力的证据，表明端粒酶活性是癌症诊断的重要标志。本研究检测了12例良性涎腺肿瘤（8例多形性腺瘤、4例腺淋巴瘤）、24例恶性肿瘤（15例粘液表皮样癌、6例腺样囊性癌、3例腺泡细胞癌）和6例非肿瘤性涎腺的端粒酶活性。还检测了人类端粒酶逆转录酶（hTERT） mRNA表达和其他端粒酶相关蛋白（hTEP1, p23， Hsp90和dyskerin）。在24例恶性肿瘤中，15例显示端粒酶活性强。非肿瘤性唾液腺端粒酶表现为阴性。此外，端粒酶活性在高级别黏液表皮样癌中显著高于低级别黏液表皮样癌(学生t检验，p
Approximately 85% of human malignant tumors express increased levels of telomerase. The marked association of telomerase activity with malignant tissue provides strong evidence that telomerase activity is a significant marker for the diagnosis of cancer. In this study, telomerase activity was examined in 12 benign salivary gland tumors (8 pleomorphic adenomas and 4 adenolymphomas), 24 malignant tumors (15 mucoepidermoid carcinomas, 6 adenoid cystic carcinomas and 3 acinic cell carcinomas) and 6 nonneoplastic salivary glands. The mRNA expression of the human telomerase reverse transcriptase (hTERT) and additional telomerase-associated proteins (hTEP1, p23, Hsp90 and dyskerin) was also examined. Of the 24 malignant tumors, 15 revealed strong telomerase activity. The non-neoplastic salivary glands appeared to have a negative telomerase expression. Furthermore, telomerase activity was significantly higher in high-grade mucoepidermoid carcinomas compared to low-grade ones (Student's t-test, p