Chitosan Oligosaccharide Reduces Intestinal Inflammation That Involves Calcium-Sensing Receptor (CaSR) Activation in Lipopolysaccharide (LPS)-Challenged Piglets
Chitosan Oligosaccharide Reduces Intestinal Inflammation That Involves Calcium-Sensing Receptor (CaSR) Activation in Lipopolysaccharide (LPS)-Challenged Piglets
复制标题
壳寡糖可减少脂多糖 (LPS) 挑战仔猪中涉及钙敏感受体 (CaSR) 激活的肠道炎症
DOI：
10.1021/acs.jafc.5b05195
复制
发表时间：
2016-01-13
影响因子：
6.1
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
Huang, Bo;Xiao, Dingfu;Yin, Yulong
壳聚糖低聚糖（COS）是壳聚糖的降解产物，具有抗氧化、抗炎、抗菌等作用。本研究采用脂多糖（LPS）应激仔猪模型，探讨饲粮中添加COS对肠道炎症反应及钙敏感受体（CaSR）和核转录因子κ B （nf - κ B）信号通路的影响。试验采用2 × 2因子设计，共选用40头断奶仔猪；主要影响因素为饲粮处理（基础或300 μ g/kg COS）和炎症刺激（LPS或生理盐水）。在治疗开始后的第14天和第21天上午，分别腹腔注射60和80 μ g/kg体重的大肠杆菌LPS或等量的无菌生理盐水。第14天和第21天分别取血和小肠标本。结果表明，LPS攻毒仔猪平均日增重、饲料增重显著降低，空肠和回肠组织病理损伤显著降低，而饲粮中添加COS可显著减轻LPS致肠道损伤。与基础饲粮相比，COS饲粮的血清肿瘤坏死因子α (tnf - α)、白细胞介素6 （IL - 6）和IL-8浓度较低，肠道促炎细胞因子mRNA丰度较低，抗炎细胞因子mRNA丰度较高（p < 0.05）。饲粮COS提高了生理盐水和脂多糖处理仔猪肠道CaSR和PLC β 2蛋白的表达，降低了脂多糖处理仔猪p- nf - κ B p65、IKK α / β和I κ B蛋白的表达（p < 0.05）。这些发现表明，COS具有降低肠道炎症反应的潜力，这与炎症刺激下CaSR的激活和NF-kappa B信号通路的抑制是同时存在的。
Chitosan oligosaccharide (COS) is a degradation product of chitosan with antioxidative, anti-inflammatory, and antibacterial effects. This study was conducted to investigate the effects of dietary COS on the intestinal inflammatory response and the calcium-sensing receptor (CaSR) and nuclear transcription factor kappa B (NF-kappa B) signaling pathways that may be involved using a lipopolysaccharide (LPS)-challenged piglet model. A total of 40 weaned piglets were used in a 2 X 2 factorial design; the main factors were dietary treatment (basal or 300 mu g/kg COS) and inflammatory challenge (LPS or saline). On the morning of days 14 and 21 after the initiation of treatment, the piglets were injected intraperitoneally with Escherichia colt LPS at 60 and 80 mu g/kg body weight or the same amount of sterilized saline, respectively. Blood and small intestine samples were collected on day 14 or 21, respectively. The results showed that piglets challenged with LPS have a significant decrease in average daily gain and gain:feed and histopathological injury in the jejunum and ileum, whereas dietary supplementation with COS significantly alleviated intestinal injury induced by LPS. Piglets fed the COS diet had lower serum concentrations of tumor necrosis factor alpha (TNF-alpha), interleukin (IL) 6, and IL-8 as well as lower intestinal abundances of pro-inflammatory cytokine mRNA but higher anti-inflammatory cytokine mRNA compared with piglets fed the basal diet among LPS-challenged piglets (p < 0.05). Dietary COS increased intestinal CaSR and PLC beta 2 protein expressions in both saline- and LPS-treated piglets, but decreased p-NF-kappa B p65, IKK alpha/beta, and I kappa B protein expressions in LPS-challenged piglets (p < 0.05). These findings indicate that COS has the potential to reduce the intestinal inflammatory response, which is concomitant with the activation of CaSR and the inhibition of NF-kappa B signaling pathways under an inflammatory stimulus.