mRNA retroposition in human cells: Processed pseudogene formation
mRNA retroposition in human cells: Processed pseudogene formation
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DOI：
10.1002/j.1460-2075.1995.tb00324.x
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发表时间：
1995-12-15
期刊：
影响因子：
11.4
通讯作者：
Heidmann, T
中科院分区：
文献类型：
--
作者：
Maestre, J;Tchenio, T;Heidmann, T
使用灵敏的逆转录事件检测方法，我们证明人类 HeLa 细胞可以以低但可检测的频率“逆转录”，即逆转录和整合初始报告基因的 mRNA。此外，我们还表明逆转录拷贝具有哺乳动物基因组中自然发现的加工假基因的所有特征：它们缺乏内含子和 5' 启动子序列，它们获得了 3' Poly(A) 尾，并且它们的两侧是短的重复（
Using a sensitive assay for detection of reverse transcription events, we demonstrate that human HeLa cells can 'retropose', i.e. reverse transcribe and integrate, the mRNA of a naive reporter gene, at a low but detectable frequency, Furthermore, we show that the retroposed copies have all the hallmarks of the processed pseudogenes naturally found in the mammalian genome: they lack intron and 5' promoter sequence, they have acquired a 3' poly(A) tail, and they are flanked by short repeats (