Lack of detection of SARS-CoV-2 in wildlife from Kerala, India in 2020-21
Lack of detection of SARS-CoV-2 in wildlife from Kerala, India in 2020-21
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2020-21 年印度喀拉拉邦野生动物中未检测到 SARS-CoV-2
DOI：
10.1099/acmi.0.000686.v3
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发表时间：
2024
影响因子：
--
通讯作者：
Zachariah A
中科院分区：
文献类型：
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作者：
Zachariah A
SARS-CoV-2 扩散到各种野生和家养动物一直是人类大流行的一个持续特征。北美白尾鹿体内建立了一个新的宿主，并且白尾鹿体内传播的病毒与人群中传播的病毒的差异日益加大，凸显了这对全球健康构成的持续风险。世界一些地区对野生动物物种进行了更密集的 SARS-CoV-2 和相关冠状病毒监测，但地理和物种特异性信息仍然存在很大差距。本文报告了使用泛冠状病毒终点 RDRP PCR 和 Sarbecovirus 特异性 E 基因 qPCR 对印度喀拉拉邦野生动物的肺和/或肠道组织进行的基于 SARS-CoV-2 PCR 的检测的阴性结果。这些动物包括：121只Rhinolophus rouxii（红马蹄蝠）、6只Rhinolophus bedommei（小毛马蹄蝠）、15只Rossettus leschenaultii（黄果蝠）、47只猕猴（Bonnet macaques）、35只Paradoxurus hermaphroditus（果子狸）、5只小灵猫indica（小印度灵猫）、4 Herpestes edwardsii（普通猫鼬）、10 Panthera tigris（孟加拉虎）、8 Panthera pardus fusca（印度豹）、4 Prionailurus bengalensis（豹猫）、2 Felis chaus（丛林猫）、2 Cuon alpinus（野狗）和 1 Melursus ursinus（懒熊）。
Spillover of SARS-CoV-2 into a variety of wild and domestic animals has been an ongoing feature of the human pandemic. The establishment of a new reservoir in white-tailed deer in North America and increasing divergence of the viruses circulating in them from those circulating in the human population has highlighted the ongoing risk this poses for global health. Some parts of the world have seen more intensive monitoring of wildlife species for SARS-CoV-2 and related coronaviruses but there are still very large gaps in geographical and species-specific information. This paper reports negative results for SARS-CoV-2 PCR based testing using a pan coronavirus end point RDRP PCR and a Sarbecovirus specific E gene qPCR on lung and or gut tissue from wildlife from the Indian State of Kerala. These animals included: 121Rhinolophus rouxii(Rufous Horsehoe Bat),sixRhinolophus bedommei(Lesser Woolly Horseshoe Bat),15Rossettus leschenaultii(Fulvous Fruit Bat),47Macaca radiata(Bonnet macaques),35Paradoxurus hermaphroditus(Common Palm Civet),fiveViverricula indica(Small Indian Civet), fourHerpestes edwardsii(Common Mongoose),tenPanthera tigris(Bengal Tiger),eightPanthera pardus fusca(Indian Leopard), fourPrionailurus bengalensis(Leopard cats), twoFelis chaus(Jungle cats), twoCuon alpinus(Wild dogs) and oneMelursus ursinus(sloth bear).