Acute Lung Injury in Response to Intratracheal Instillation of Lipopolysaccharide in an Animal Model of Emphysema Induced by Elastase
Acute Lung Injury in Response to Intratracheal Instillation of Lipopolysaccharide in an Animal Model of Emphysema Induced by Elastase
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DOI：
10.1007/s10753-017-0675-5
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发表时间：
2018-02-01
期刊：
影响因子：
5.1
通讯作者：
Pinheiro, Bruno Valle
中科院分区：
文献类型：
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作者：
Esteves Rabelo, Maria Aparecida;Fonseca Lucinda, Leda Marilia;Pinheiro, Bruno Valle
肺气肿肺对急性肺损伤 (ALI) 的反应仍不清楚。本研究比较了患有和不患有肺气肿的大鼠对气管内滴注脂多糖（LPS）的肺部反应。将24只Wistar大鼠随机分为4组：对照组（C-G）、ALI组（ALI-G）、肺气肿组（E-G）、肺气肿ALI组（E-ALI-G）。 ALI诱导后24小时进行安乐死和以下分析：肺组织学、支气管肺泡灌洗(BAL)、炎症介质的mRNA表达和血气测量。组织学分析显示，ALI-G (0.55 +/- 0.15) 和 E-ALI-G (0.69 +/- 0.08) 动物的 ALI 评分高于 C-G (0.12 +/- 0.04) 和 E-G (0.16 +/- 0.04) (p < 0.05)。对评分各组成部分的分析表明，ALI-G 和 E-ALI-G 具有更大的肺泡和间质中性粒细胞浸润，以及更多的肺泡蛋白质碎片。比较接受 LPS 的两组，E-ALI-G 中存在较高 ALI 的趋势，特别是由于肺泡腔中中性粒细胞浸润较高和间隔增厚较高。总细胞计数（E-G = 3.09 +/- 0.83；ALI-G = 4.45 +/- 1.9；E-ALI-G = 5.9 +/- 2.1；C-G = 0.73 +/- 0.37 x 10(5)）和中性粒细胞计数（E-G = 0.69 +/- 0.35；ALI-G = 2.53 +/-与 C-G 相比，E-G、ALI-G 和 E-ALI-G 组的 BAL 值较高（p < 0.05；E-ALI-G = 1.09；E-ALI-G = 3.86 +/- 1.4；C-G = 0.09 +/- 0.07 x 10(5)）。与 C-G 和 E-G 组相比，接受 LPS（ALI-G 和 E-ALI-G）的动物中 IL-6、TNF-α 和 CXCL2 mRNA 表达较高（p < 0.05）。 ALI-G 和 E-ALI-G 之间的 BAL 细胞结构和炎症介质表达没有观察到统计学上的显着差异。在患有和不患有气管内诱发性肺气肿的大鼠中，气管内滴注 LPS 引起的 ALI 严重程度没有表现出差异。
The response of lungs with emphysema to an acute lung injury (ALI) remains unclear. This study compared the lung response to intratracheal instillation of lipopolysaccharide (LPS) in rats with and without emphysema. Twenty-four Wistar rats were randomized to four groups: control group (C-G), ALI group (ALI-G), emphysema group (E-G), emphysema and ALI group (E-ALI-G). Euthanasia and the following analysis were performed 24 h after ALI induction: lung histology, bronchoalveolar lavage (BAL), mRNA expression of inflammatory mediators, and blood gas measures. The histological analysis showed that animals of ALI-G (0.55 +/- 0.15) and E-ALI-G (0.69 +/- 0.08) had a higher ALI score compared to C-G (0.12 +/- 0.04) and E-G (0.16 +/- 0.04) (p < 0.05). The analysis of each component of the score demonstrated that ALI-G and E-ALI-G had greater alveolar and interstitial neutrophil infiltration, as well as greater amount of alveolar proteinaceous debris. Comparing the two groups that received LPS, there was a trend of higher ALI in the E-ALI-G, specially due to a higher neutrophil infiltration in the alveolar spaces and a higher septal thickening. Total cell count (E-G = 3.09 +/- 0.83; ALI-G = 4.45 +/- 1.9; E-ALI-G = 5.9 +/- 2.1; C-G = 0.73 +/- 0.37 x 10(5)) and neutrophil count (E-G = 0.69 +/- 0.35; ALI-G = 2.53 +/- 1.09; E-ALI-G = 3.86 +/- 1.4; C-G = 0.09 +/- 0.07 x 10(5)) in the BAL were higher in the groups E-G, ALI-G, and E-ALI-G when compared to C-G (p < 0.05). The IL-6, TNF-alpha, and CXCL2 mRNA expressions were higher in the animals that received LPS (ALI-G and E-ALI-G) compared to the C-G and E-G (p < 0.05). No statistically significant difference was observed in the BAL cellularity and in the expression of inflammatory mediators between the ALI-G and the E-ALI-G. The severity of ALI in response to intratracheal instillation of LPS did not show difference in rats with and without intratracheal-induced emphysema.