Chromosomal DNA probes for the identification of Bacteroides species.
Chromosomal DNA probes for the identification of Bacteroides species.
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用于鉴定拟杆菌属物种的染色体 DNA 探针。
DOI：
10.1099/00221287-133-6-1423
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Kenny,GE
中科院分区：
文献类型：
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作者：
Roberts,MC;Moncla,B;Kenny,GE
我们用S1内切酶方法对22个类杆菌进行了DNA-DNA同源性研究。目前定义的物种与除B以外的任何其他物种的DNA同源性都没有超过30%。Boccae和B。头状菌(与b.它们现在被认为是一个单一的物种)，它们拥有86%的DNA序列。两个类群的亲缘关系较弱，DNA同源性为10%。第一个群包括分贝。Corporation、B.disiens、B.bivius、B.medius和B.黑色素生成。第二个群包括分贝。Fragilis、卵形双胞菌B.eggerthii、卵形双胞菌B.thetaiotaomicronandB.制服。5个口腔种，B.asaccharolyticus，B.gigivalis，B.loescheii，B.medius和B.在斑点印迹分析中，以产黑线虫为研究对象进行了全染色体探针的研究。对243株生化鉴定为类杆菌的临床菌株进行了测试。DNA探针正确识别了94%的临床菌株。DNA探针和生化鉴定5种中有2种为100%。相比之下，只有86%的菌株进行了生化鉴定。通过DNA探针鉴定了中间体。DNA探针对7株无法进行生化鉴定的菌株进行了物种鉴定。
We compared 22Bacteroidesspecies by DNA-DNA homology studies using the S1 endonuclease method. None of the currently defined species shared more than 30% DNA homology with any other species examined with the exception ofB. buccaeandB. capillus(which along withB. pentosaceusare now considered a single species), which shared 86% of their DNA sequences. Two clusters showed weak genetic relationships, with DNA homology > 10%. The first cluster includedB. corporis, B. disiens, B. bivius, B. intermediusandB. melaninogenicus. The second cluster includedB. fragilis, B. eggerthii, B. ovatus, B. thetaiotaomicronandB. uniformis. Five of the oral species,B. asaccharolyticus, B. gingivalis, B. loescheii, B. intermediusandB. melanogenicus, were chosen for study as whole chromosomal probes in dot blot assays. These were tested against 243 clinical strains biochemically identified asBacteroidesspecies. The DNA probes correctly identified 94% of the clinical strains. DNA probe and biochemical identification was 100% for two of the five species. In contrast, only 86% of the strains biochemically identified asB. intermediuswere identified by the DNA probe. The DNA probes gave a species identification to seven strains which could not be biochemically identified.