MYOCARDIAL FIBER MAPPING OF RAT HEARTS, USING APPARENT BACKSCATTER, WITH HISTOLOGIC VALIDATION
MYOCARDIAL FIBER MAPPING OF RAT HEARTS, USING APPARENT BACKSCATTER, WITH HISTOLOGIC VALIDATION
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DOI：
10.1016/j.ultrasmedbio.2019.05.002
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发表时间：
2019-08-01
影响因子：
2.9
通讯作者：
Chung, Charles S.
中科院分区：
文献类型：
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作者：
Milne, Michelle L.;Schick, Brianna M.;Chung, Charles S.
心肌纤维结构是射血分数、应变和压力发展的重要生理调节因子。表观超声背向散射已被证明是一种有用的方法，用于重建心肌纤维结构在人类大小的绵羊心脏，因为它的振幅的依赖于相对方向的肌纤维的角度的超声声穿透。因此，反向散射信号的各向异性与纤维取向相关并提供关于纤维取向的信息。在这项研究中，我们试图确定表观背散射是否可用于测量啮齿动物心脏中的肌纤维取向。固定的成年大鼠心脏完整成像，并对左心室游离壁的透壁圆柱形核心和透壁楔形进行成像。圆柱形核心样品证实，反向散射各向异性可以在大鼠心脏测量。透壁心肌楔形样本的超声和组织学分析证实，明显的背向散射可以再现映射到纤维方向（纤维相对于声穿透方向的角度）。这些数据提供了一个定量关系的表观背向散射和纤维角，适用于整个心脏的图像。成功地测量了大鼠心脏的心肌纤维结构。使用明显的反向散射定量心肌纤维结构提供了许多优点，包括其从啮齿动物到人类的可扩展使用，其快速低成本采集和最小的禁忌症。本研究中概述的方法为研究人员提供了一种方法，可以开始详细评估临床前疾病模型中心肌纤维结构的变化，并可以立即转化为临床。(C)2019作者（S）爱思唯尔公司出版代表世界超声波医学与生物学联合会。
Myocardial fiber architecture is a physiologically important regulator of ejection fraction, strain and pressure development. Apparent ultrasonic backscatter has been shown to be a useful method for recreating the myocardial fiber architecture in human-sized sheep hearts because of the dependence of its amplitude on the relative orientation of a myofiber to the angle of ultrasonic insonification. Thus, the anisotropy of the backscatter signal is linked to and provides information about the fiber orientation. In this study, we sought to determine whether apparent backscatter could be used to measure myofiber orientation in rodent hearts. Fixed adult-rat hearts were imaged intact, and both a transmural cylindrical core and transmural wedge of the left ventricular free wall were imaged. Cylindrical core samples confirmed that backscatter anisotropy could be measured in rat hearts. Ultrasound and histologic analysis of transmural myocardial wedge samples confirmed that the apparent backscatter could be reproducibly mapped to fiber orientation (angle of the fiber relative to the direction of insonification). These data provided a quantitative relationship between the apparent backscatter and fiber angle that was applied to whole-heart images. Myocardial fiber architecture was successfully measured in rat hearts. Quantifying myocardial fiber architecture, using apparent backscatter, provides a number of advantages, including its scalable use from rodents to man, its rapid low-cost acquisition and minimal contraindications. The method outlined in this study provides a method for investigators to begin detailed assessments of how the myocardial fiber architecture changes in preclinical disease models, which can be immediately translated into the clinic. (C) 2019 The Author(s). Published by Elsevier Inc. on behalf of World Federation for Ultrasound in Medicine & Biology.