How does osteocalcin lacking γ-glutamic groups affect biomimetic apatite formation and what can we say about its structure in mineral-bound form?
How does osteocalcin lacking γ-glutamic groups affect biomimetic apatite formation and what can we say about its structure in mineral-bound form?
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DOI：
10.1016/j.jsb.2019.04.014
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发表时间：
2019-08-01
影响因子：
3
通讯作者：
Goobes, Gil
中科院分区：
文献类型：
--
作者：
Iline-Vul, Taly;Kulpanovich, Alexey;Goobes, Gil
骨钙素等非胶原蛋白充当骨矿化过程的调节剂。骨钙素经过翻译后修饰，在其三个谷氨酸残基上添加额外的羧酸基团，以增强与骨矿物质的相互作用。在这项工作中，我们研究了翻译后未修饰的骨钙素对仿生磷灰石形成的调节。我们分析蛋白质和矿物质-蛋白质界面的结构特征，以引出矿物质内部未修饰的蛋白质折叠，并揭示与矿物质表面相互作用的物种。这里给出的结果提供了关于该蛋白质在控制羟基磷灰石晶体上形成的矿物相中的积极作用及其影响这些晶体的有序程度的能力的线索。
Non-collagenous proteins such as osteocalcin function as regulators of the mineralization process in bone. Osteocalcin undergoes post-translational modification adding an extra carboxylate group on three of its glutamate residues to enhance interaction with bone mineral. In this work, we examine regulation of biomimetic apatite formation by osteocalcin that was not modified after translation. We analyze the structural features in the protein and mineral-protein interfaces to elicit the unmodified protein's fold inside the mineral and to unveil the species that interact with the mineral surface. The results presented here give clues on the protein's active role in controlling the mineral phases that are formed on hydroxyapatite crystals and its ability to influence the extent of order in these crystals.