THE RECOVERY OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES FROM SUBLYTIC COMPLEMENT ATTACK IS MEDIATED BY CHANGES IN INTRACELLULAR FREE CALCIUM
THE RECOVERY OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES FROM SUBLYTIC COMPLEMENT ATTACK IS MEDIATED BY CHANGES IN INTRACELLULAR FREE CALCIUM
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DOI：
10.1042/bj2310205
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
CAMPBELL, AK
中科院分区：
文献类型：
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作者：
MORGAN, BP;CAMPBELL, AK
利用多态核白细胞-红细胞鬼影杂合体包裹钙活化的光蛋白obelin，我们已经证明了补体膜攻击复合物的亚溶解量诱导细胞内游离钙离子浓度（[Ca2+]i）的快速但短暂的增加。这种[Ca2+]i的增加发生在细胞膜攻击复合物从细胞表面快速去除之前，并且是必需的。[Ca2+]i的增加不仅是由于细胞外的内流增加，而且是由于细胞内储存的动员。讨论了钙动员的可能机制，以及[Ca2+]i作为有核细胞恢复过程介质的重要性。
Using polymorphonuclear leucocyte-erythrocyte ghost hybrids entrapping the calcium-activated photoprotein obelin, we have demonstrated that sublytic amounts of the complement membrane attack complex induce a rapid but transient increase in intracellular free calcium ion concentration ([Ca2+]i). This increase in [Ca2+]i occurs prior to, and is required for, rapid removal of membrane attack complexes from the cell surface. The increase in [Ca2+]i is not only due to increased influx from outside the cell, but also results from mobilization of intracellular stores. The possible mechanism of mobilization of calcium, and the importance of an increase in [Ca2+]i as a mediator of recovery processes in nucleated cells, are discussed.