A purified green barley extract with modulatory properties upon TNF alpha and ROS released by human specialised cells isolated from RA patients.
A purified green barley extract with modulatory properties upon TNF alpha and ROS released by human specialised cells isolated from RA patients.
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发表时间：
1998-07
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通讯作者：
L. Cremer;A. Herold;D. Avram;G. Szegli
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作者：
L. Cremer;A. Herold;D. Avram;G. Szegli
基于活性氧（ROS）在介导促炎细胞因子（如TNF α）产生中起重要作用的发现，以及许多植物提取物含有具有抗氧化特性的物质，我们研究了绿大麦提取物的抗炎作用，市售的“天然SOD”。本研究结果表明，“天然SOD”的抗炎特性是由于其微分子物质，能够清除ROS并下调TNF α的产生，TNF α是类风湿性关节炎（RA）患者外周血（PB）和滑液（SF）中特化细胞产生的主要炎症介质。我们制备并测试了一种纯化的绿大麦提取物（PE），该提取物含有1 kDa以下的微分子物质，能够抑制从RA患者的PB和SF中分离的LPS刺激的人单个核细胞（MNC）释放TNF α。鲁米诺依赖性化学发光已被用于测量PE对RA患者PB中活化中性粒细胞释放的ROS的清除活性。PE含有高浓度的抗氧化和抗炎物质，在治疗RA患者方面可能是一种比“天然SOD”更有效的天然药物。
Based on the finding that reactive oxygen species (ROS) play important roles in mediating proinflammatory cytokine production (e.g. TNF alpha) and that many plant extracts contain substances with antioxidant properties, we examined the antiinflammatory action of a green barley extract, commercially available as "Natural SOD". The results obtained in this study demonstrate that the antiinflammatory properties of "Natural SOD" due to its micromolecular substances, able to scavenge ROS and to down-regulate TNF alpha production, main inflammation mediators produced by specialised cells from peripheral blood (PB) and synovial fluid (SF) of patients with rheumatoid arthritis (RA). We prepared and tested a purified green barley extract (PE) containing micromolecular substances under 1 kDa, able to inhibit TNF alpha releasing--measured by an bioassay--from LPS stimulated human mononuclear cells (MNC) isolated both from PB and SF of RA patients. Luminol-dependent chemiluminescence has been used to measure the scavenging activity of PE on ROS releasing from activated neutrophils isolated from PB of RA patients. PE, containing high concentrations of substances with antioxidant and antiinflammatory properties, could be a more efficient natural drug for human use than "Natural SOD" in the treatment of RA patients.