IMMUNOREACTIVE DYNORPHIN IN HUMAN-BRAIN AND PITUITARY
IMMUNOREACTIVE DYNORPHIN IN HUMAN-BRAIN AND PITUITARY
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DOI：
10.1016/0006-8993(82)90930-1
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发表时间：
1982-01-01
期刊：
影响因子：
2.9
通讯作者：
HERZ, A
中科院分区：
文献类型：
--
作者：
GRAMSCH, C;HOLLT, V;HERZ, A
用高特异性放射免疫法测定了强啡肽(ir-dyn)免疫反应阳性物质在人脑和垂体不同部位的分布。在所研究的26个脑区中，黑质(24.5pmol/g)和下丘脑的ir-dyn浓度最高。在其他锥体外结构，如尾状核、苍白球和壳核，也可测量到大量的数量。Ir-dyn在杏仁核、海马体、中脑导水管周围灰质、丘脑、桥脑、延髓和最后区有较低浓度的表达，在垂体后叶有少量表达，在前叶未检测到Ir-dyn。凝胶渗透层析显示，脑内免疫反应性由3-4个apprx的表观分子量峰组成。12,000,6000,1800和<；1000。其中两个峰经免疫沉淀纯化后，在豚鼠回肠纵肌生物测定中显示出较高的阿片效价。Ir-dyn的分布模式与脑啡肽有一些相似之处，而Ir-β-内啡肽的分布则截然不同。
The distribution of immunoreactive dynorphin (ir-dyn) was determined in various regions of human brain and pituitary by use of a highly specific radioimmunoassay. The concentrations of ir-dyn in the substantia nigra (24.5 pmol/g) and hypothalamus were among the highest in the 26 brain areas examined. Substantial amounts were also measurable in other extrapyramidal structures such as the caudate nucleus, pallidus and putamen. Lower concentrations of ir-dyn were detected in the amygdala, hippocampus, periaqueductal gray matter, colliculi, pons, medulla and area postrema, but only low amounts were found in the posterior lobe of the pituitary, while no ir-dyn was detectable in the anterior lobe. By gel permeation chromatography the brain immunoreactivity was shown to consist of 3-4 peaks of apparent MW of .apprx. 12,000, 6000, 1800 and < 1000. High opioid potency of 2 of these peaks was demonstrated in the guinea pig ileum longitudinal muscle bioassay after purification by immunoprecipitation. A comparison of the distribution pattern of ir-dyn revealed some parallels with enkephalin, whereas the distribution of ir-.beta.-endorphin is quite different.