Characterisation and expression analysis of an interleukin 6 hornologue in the Japanese pufferfish, Fugu rubripes
Characterisation and expression analysis of an interleukin 6 hornologue in the Japanese pufferfish, Fugu rubripes
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DOI：
10.1016/j.dci.2005.01.002
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Secombes, C
中科院分区：
文献类型：
--
作者：
Bird, S;Zou, J;Secombes, C
通过利用人类和河豚基因组的某些区域之间的同源性，在河豚中确定了鱼类中的第一个IL-6序列。预测的227aa IL-6同源物包含IL-6/G-CSF/MGF基序，具有预测的四个α -螺旋的二级结构，但只包含在二硫键形成中重要的四种半胱氨酸中的两种。尽管系统发育分析将河豚分子与其他IL-6分子归为一类，但它与已知IL-6序列的氨基酸同源性较低（20-29%）。河豚IL-6的基因结构和人类与河豚基因组的同源性在进化过程中得到了很好的保守，基因的顺序和取向与人类7号染色体完全匹配。PHA刺激河豚肾细胞导致河豚IL-6转录物大量增加，而LPS和Poly I:C导致脾脏细胞内IL-6转录物显著增加。鱼类中IL-6的发现现在可以更详细地调查局部炎症反应。(c) 2005 Elsevier Ltd版权所有。
The first IL-6 sequence in fish has been determined in Fugu rubripes by exploiting the synteny that is found between some regions of the human and Fugu genomes. The predicted 227 aa IL-6 homologue contains the IL-6/G-CSF/MGF motif, has a predicted secondary structure of four alpha-helixes but only contains two of the four cysteines important in disulphide bond formation. It shows low amino acid identities (20-29%) with known IL-6 sequences, although phylogenetic analysis groups the Fugu molecule with the other IL-6 molecules. The gene organisation of Fugu IL-6 and the level of synteny between the human and Fugu genomes has been well conserved during evolution with the order and orientation of the genes matching exactly to human chromosome 7. PHA stimulation of Fugu kidney cells resulted in a large increase in the Fugu IL-6 transcript, whereas LPS and Poly I:C resulted in a significant increase within spleen cells. The discovery of IL-6 in fish will now allow more detailed investigations of local inflammatory responses. (c) 2005 Elsevier Ltd. All rights reserved.