Rapid isolation of single malaria parasite-infected red blood cells by cell sorting
Rapid isolation of single malaria parasite-infected red blood cells by cell sorting
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DOI：
10.1038/nprot.2010.185
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发表时间：
2011-02-01
期刊：
影响因子：
14.8
通讯作者：
Cui, Liwang
中科院分区：
文献类型：
--
作者：
Miao, Jun;Cui, Liwang
Malaria research often requires isolation of individually infected red blood cells (RBCs) or of a homogenous parasite population derived from a single parasite (clone). Traditionally, isolation of individual, parasitized RBCs or parasite cloning is achieved by limiting dilution or micromanipulation. This protocol describes a method for more efficient cloning of the malaria parasite; the method uses a cell sorter to rapidly isolate Plasmodium falciparum-infected RBCs singly. By gating the parameters of forward-angle light scatter and side-angle light scatter in a cell sorter, singly infected RBCs can be isolated and automatically deposited into a 96-well culture plate within 1 min. Including a Percoll purification step; the entire procedure to seed a 96-well plate with singly infected RBCs can take
