Oxidored-nitro domain containing protein 1 (NOR1) expression suppresses slug/vimentin but not snail in nasopharyngeal carcinoma: Inhibition of EMT in vitro and in vivo in mice
Oxidored-nitro domain containing protein 1 (NOR1) expression suppresses slug/vimentin but not snail in nasopharyngeal carcinoma: Inhibition of EMT in vitro and in vivo in mice
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鼻咽癌中含有氧化硝基结构域的蛋白 1 (NOR1) 表达抑制 slug/vimentin，但不抑制 snail：小鼠体外和体内 EMT 抑制
DOI：
10.1016/j.canlet.2014.03.005
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发表时间：
2014-06-28
期刊：
影响因子：
9.7
通讯作者：
Li, Guiyuan
中科院分区：
文献类型：
--
作者：
Wang, Wei;Li, Xiaoling;Li, Guiyuan
含氧化硝基结构域蛋白1（NOR 1）是一种公认的肿瘤抑制基因。本研究检测了NOR 1在鼻咽癌组织和非癌鼻咽上皮中的表达。结果表明，NOR 1蛋白在鼻咽癌组织中表达减少。NOR 1蛋白表达缺失与鼻咽癌患者的总体生存率和无事件生存率相关。NOR 1在NPC细胞中的过表达导致显著的形态学变化和上皮-间充质转化（EMT）介质（例如，蛞蝓和波形蛋白），但诱导细胞角蛋白13的表达。裸鼠体内转移实验表明，NOR 1的过表达降低了鼻咽癌细胞的体内转移能力。敲低HeLa细胞中的NOR 1表达足以废除上皮性状并增强细胞迁移和侵袭。同时抑制蛞蝓或波形蛋白减轻诱导EMT，迁移或入侵的NOR 1 siRNA在HeLa细胞在体外。总之，本研究的数据首次表明，NOR 1在离体、体外和体内NPC中起重要作用。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Oxidored-nitro domain containing protein 1 (NOR1) is a putative tumor suppressor gene. In this study, NOR1 expression was detected in NPC tissues and non-cancerous nasopharyngeal epithelium. The data showed that NOR1 protein was decreased in NPC tissues. Lost expression NOR1 protein was associated with poor overall and event-free survival of NPC patients. Overexpression of NOR1 in NPC cells resulted in a significant morphological change and decreased expression of epithelial-to-mesenchymal transition (EMT) mediators (e.g., slug and vimentin), but induced cytokeratin 13 expression. A nude mouse metastasis assay revealed that overexpression of NOR1 decreased NPC tumor cells metastasis capacity in vivo. Knockdown of NOR1 expression in HeLa cells was sufficient to abrogate epithelial traits and to enhance cell migration and invasion. Concomitant inhibition of slug or vimentin alleviated induction of EMT, migration or invasion by NOR1 siRNA in HeLa cells in vitro. In conclusion, the data from the current study suggest, for the first time, that NOR1 plays an important role in NPC in ex vivo, in vitro, and in vivo. (C) 2014 Elsevier Ireland Ltd. All rights reserved.