Resynthesis of insulin from its A and B chains in the presence of denaturants.
Resynthesis of insulin from its A and B chains in the presence of denaturants.
复制标题
在变性剂存在下从 A 链和 B 链重新合成胰岛素。
DOI：
10.1016/0006-291x(87)90548-1
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Tsou,CL
中科院分区：
文献类型：
--
作者：
Qian,YQ;Tsou,CL
还原的胰岛素A和B链在8 M尿素中的再氧化导致2-7%的天然胰岛素回收，而在6 M胍或0.5 mM十二烷基硫酸盐中，可以检测到非常少的胰岛素再合成（如果有的话）。考虑到含有一条或两条链的不同低聚物形式的所有可能性，8 M尿素的产率远高于由链的随机连接计算的产率，胍或十二烷基硫酸盐的产率是预期的。它的结论是，一些相互作用和配对的链发生，即使在8 M尿素的存在下。
Reoxidation of the reduced insulin A and B chains in 8 M urea leads to a recovery of native insulin of 2–7% whereas in 6 M guanidine or 0.5 mM dodecylsulfate very little, if any, resynthesis of insulin could be detected. Considering all the possibilities of different oligomeric forms containing one or both of the chains, the yield in 8 M urea is well over the yield as calculated from random joining of the chains and the yield in guanidine or dodecylsulfate is to be expected. It is concluded that some interaction and pairing of the chains occur even in the presence of 8 M urea.