Genetic control of multiple pathways of post-replicational repair in uvrB strains of Escherichia coli K-12
Genetic control of multiple pathways of post-replicational repair in uvrB strains of Escherichia coli K-12
复制标题
大肠杆菌 K-12 uvrB 菌株复制后修复多种途径的遗传控制
作者：
D. A. Youngs;K. Smith
研究了recA、uvrD、exrA和recB突变以及氯霉素照射后处理对大肠杆菌K-12 uvrB菌株紫外线照射后的存活和复制后修复的影响。发现这些突变或治疗中的每一种都会降低存活率和修复程度。通过检查几种多重突变体的存活和修复特征来确定uvrD、exaA和recB突变的抑制作用与氯霉素治疗的相互作用。存活结果表明uvrB菌株的复制后修复过程可能被细分为至少五个不同的分支。其中包括被 exrA、recB 或 uvrD 突变阻断的三个分支，被任何这些突变阻断且对氯霉素治疗敏感的第四个分支，以及至少一个对这些突变或氯霉素治疗不敏感的附加分支。每种菌株观察到的复制后修复程度与基于存活数据假设的途径大体一致，尽管这种相关性存在一些明显的例外。
The effect of the recA, uvrD, exrA, and recB mutations and of post-irradiation treatment with chloramphenicol on the survival and post-replication repair after ultraviolet irradiation of uvrB strains of Escherichia coli K-12 was examined. Each of these mutations or treatments was found to decrease survival and the extent of repair. The interactions of the inhibitory effects of the uvrD, exaA, and recB mutations and chloramphenicol treatment were determined by examining the survival and repair characteristics of the several multiple mutants. The survival results suggest that the post-replication repair process in uvrB strains may be subdivided into at least five different branches. These include three branches that are blocked by the exrA, recB, or uvrD mutation, a fourth branch that is blocked by any of these mutations and is also sensitive to chloramphenicol treatment, and at least one additional branch that is not sensitive to either of these mutations or to chloramphenicol treatment. The extent of post-replicational repair observed with each of the strains is in general agreement with the pathways postulated on the basis of the survival data, although there are several apparent exceptions to this correlation.