Search for DNA of exogenous mouse mammary tumor virus-related virus in human breast cancer samples
Search for DNA of exogenous mouse mammary tumor virus-related virus in human breast cancer samples
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DOI：
10.1099/vir.0.82767-0
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发表时间：
2007-06-01
影响因子：
3.8
通讯作者：
Blomberg, J.
中科院分区：
文献类型：
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作者：
Bindra, A.;Muradrasoli, S.;Blomberg, J.
早期关于与小鼠乳腺肿瘤病毒（MMTV）密切相关的人类外源性逆转录病毒（HMTV）的报道促使我们在乳腺癌组织和正常组织中寻找这些病毒序列。基于MMTV和已发表的HMTV包膜序列，建立了实时PCR。实时PCR方法可以检测1至10个拷贝的MMTV靶DNA。从18名乳腺癌患者和11名非恶性对照病例中前瞻性地收集组织样本，以及来自相同女性的外周血白细胞。尽管使用的实时PCR方法的高灵敏度，没有一个样品是阳性的HMTV DNA或RNA。在乳腺癌样本和对照中均不存在HMTV DNA，这表明乳腺癌中推定的HMTV DNA浓度太低而无法检测，或者它不存在。
Earlier reports of a human exogenous retrovirus (HMTV) related closely to mouse mammary tumor virus (MMTV) led us to search for these viral sequences in breast cancer tissues and normal tissues. A real-time PCR was developed based on MMTV and published HMTV envelope sequences. The real-time PCR method can detect one to ten copies of MMTV target DNA. Tissue samples were collected prospectively from 18 breast cancer patients and 11 non-malignant control cases, as well as peripheral blood leukocytes from the same women. Despite the high sensitivity of the real-time PCR method used, none of the samples were positive for HMTV DNA or RNA. The absence of HMTV DNA in both breast cancer samples and controls indicates either that the concentration of putative HMTV DNA in the breast cancers was too low for detection or that it did not exist there.