Slow wave heterogeneity within the circular muscle of the canine gastric antrum.
Slow wave heterogeneity within the circular muscle of the canine gastric antrum.
复制标题
犬胃窦环形肌内的慢波异质性。
DOI：
10.1113/jphysiol.1985.sp015793
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Sanders,KM
中科院分区：
文献类型：
--
作者：
Bauer,AJ;Reed,JB;Sanders,KM
设计了一种横截面制备，其中多个微电极可以精确定位，以刺穿从浆膜到粘膜下层的任何地方的平滑肌细胞。细胞内电记录从胃窦环行肌细胞从肌间神经丛到粘膜下层获得。静息膜电位随肌间神经丛到粘膜下层距离的变化呈线性关系。慢波upstroke dV/dt，upstroke电位振幅，和平台电位振幅线性变化的函数，从肌间神经丛到粘膜下层的距离。当慢波同时记录从一个圆形的细胞附近的肌间神经丛和粘膜下层附近的细胞，该事件总是首先发生在肌间神经丛附近的细胞。电差异似乎不是由慢波的电紧张性衰减引起的，因为它们通过环形肌肉传播。电差异不能解释的基础上的差异，内在的神经活动或前列腺素合成。膜极化不能解释肌间和粘膜下环行肌细胞慢波的差异。本文的结论是，肌间神经丛和粘膜下层附近的细胞的兴奋性机制和/或被动膜特性之间存在根本的差异。这些差异可能表现在这两个肌肉细胞群的不同运动性能。
A cross‐sectional preparation was designed in which multiple micro‐electrodes can be precisely positioned to impale smooth muscle cells anywhere from the serosa to the submucosa. Intracellular electrical recordings were obtained from gastric antral circular muscle cells from the myenteric plexus to the submucosa. The resting membrane potential changed linearly as a function of distance from the myenteric plexus to the submucosa. Slow wave upstroke dV/dt, upstroke potential amplitude, and plateau potential amplitude changed linearly as a function of distance from the myenteric plexus to the submucosa. When slow waves were recorded simultaneously from a circular cell near the myenteric plexus and from a cell near the submucosa, the event always occurred first in the cell near the myenteric plexus. Electrical differences did not appear to be caused by electrotonic decay of slow waves as they propagated through the circular muscle. Electrical differences could not be explained on the basis of differences in intrinsic neural activity or prostaglandin synthesis. Membrane polarization could not explain the differences in slow waves between myenteric and submucosal circular muscle cells. The conclusion of this paper is that fundamental differences exist between the excitability mechanisms and/or passive membrane properties of cells near the myenteric plexus and the submucosa. These differences might be manifest in different motor performance of these two muscle cell populations.