TileQC: A system for tile-based quality control of Solexa data
TileQC: A system for tile-based quality control of Solexa data
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DOI：
10.1186/1471-2105-9-250
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发表时间：
2008-05-28
影响因子：
3
通讯作者：
Denver, Dee R.
中科院分区：
文献类型：
--
作者：
Dolan, Peter C.;Denver, Dee R.
背景资料：下一代DNA测序技术，如Illumina的Solexa平台和罗氏的454方法，为研究基因组规模的问题提供了新的途径。然而，他们也提出了新的分析挑战，必须满足他们的有效应用到biological questions.Results：在这里，我们报告的可用性的瓷砖QC，瓷砖为基础的质量控制系统Solexa的数据写在R语言。TileQC提供了一种识别Solexa输出中偏差和错误的方法，通过图形表示由流动池瓦片生成的数据。图像中表示的数据然后在R环境中进行进一步分析和错误检测的自动化。结论：TileQC为基于Solexa的DNA序列数据的质量控制提供了一个高度适应性和强大的工具。
Background: Next-generation DNA sequencing technologies such as Illumina's Solexa platform and Roche's 454 approach provide new avenues for investigating genome-scale questions. However, they also present novel analytical challenges that must be met for their effective application to biological questions.Results: Here we report the availability of tileQC, a tile-based quality control system for Solexa data written in the R language. TileQC provides a means of recognizing bias and error in Solexa output by graphically representing data generated by flow cell tiles. The data represented in the images is then made available in the R environment for further analysis and automation of error detection.Conclusion: TileQC offers a highly adaptable and powerful tool for the quality control of Solexa-based DNA sequence data.