A novel embolic stroke model resembling lacunar infarction following proximal middle cerebral artery occlusion in beagle dogs
A novel embolic stroke model resembling lacunar infarction following proximal middle cerebral artery occlusion in beagle dogs
复制标题
比格犬近端大脑中动脉闭塞后类似腔隙性梗死的新型栓塞性中风模型
DOI：
10.1016/j.jneumeth.2012.06.009
复制
发表时间：
2012-07-30
影响因子：
3
通讯作者：
Shi, Hai-Bin
中科院分区：
文献类型：
--
作者：
Liu, Sheng;Hu, Wei-Xing;Shi, Hai-Bin
据估计，腔隙性脑梗死占所有缺血性脑卒中的25%，但其确切病因仍有争议。现有的争议包括栓塞是否真的会在人类或动物模型中引起腔隙性中风。我们假设，腔隙性脑梗死可以诱导近端大脑中动脉（MCA）节段性闭塞涉及的动物模型，其中有丰富的远端大脑侧支吻合的纹状体动脉的开口。本研究采用直径约1.7 mm、长约5 mm的自体血栓建立了8只比格犬大脑中动脉（MCA）近端闭塞模型，并在栓塞后6 h内每隔30 min用弥散加权成像（DWI）评价缺血病变的进展，探讨腔隙性脑梗死的可能机制。我们的结果表明，在所有犬中，使用介入性单血栓方法可以成功地闭塞左侧近端MCA。DWI显示脑深部小的单发或多发缺血性病变，术后DWI平均检出时间为1.21 ± 0.45 h，6 h-DWI直径为6.62 ± 0.60 mm。总之，我们的方法建立了一个缺血模型，可以概括的放射学和组织学变化的腔隙性脑梗死，表明栓塞可以导致腔隙性脑梗死的动物模型。(c)2012爱思唯尔有限公司版权所有。
It is estimated that lacunar infarcts account for 25% of all ischemic strokes, but its exact etiology is still on debating. The existing controversies include whether the embolisms can indeed cause lacunar stroke in humans or animal models. We hypothesized that lacunar infarction can be induced by the proximal middle cerebral artery (MCA) segmental occlusion involving the orifices of lenticulostriate arteries in animal models, which have abundant distal cerebral collateral anastomosis. Our work here establishes a proximal MCA occlusion model using thrombi (autologous blood clots about 1.7 mm in diameter and 5 mm in length) in 8 beagle dogs, evaluates the progression of ischemic lesions at 30 min interval within 6 h after embolization using the diffusion weighted imaging (DWI), and discusses the potential mechanisms of lacunar infarction. Our results indicate that the left proximal MCAs can be successfully occluded in all dogs using interventional single-thrombus method. The small solitary or multiple ischemic lesions shown in DWI were observed in the deep brain area, with the mean detecting time of 1.21 +/- 0.45 h using DWI and diameter of 6.62 +/- 0.60 mm in 6h-DWI after procedure. In conclusion, our method established an ischemic model which can recapitulate the radiologic and histologic changes in lacunar infarcts, suggesting that emboli can cause lacunar infarcts in animal model. (c) 2012 Elsevier B.V. All rights reserved.