Development and characterization of alginate coated low molecular weight chitosan nanoparticles as new carriers for oral vaccine delivery in mice
Development and characterization of alginate coated low molecular weight chitosan nanoparticles as new carriers for oral vaccine delivery in mice
复制标题
DOI：
10.1016/j.carbpol.2014.12.044
复制
发表时间：
2015-05-05
影响因子：
11.2
通讯作者：
Saha, Malay Kumar
中科院分区：
文献类型：
--
作者：
Biswas, Subrata;Chattopadhyay, Mainak;Saha, Malay Kumar
在本研究中，低分子量壳聚糖（CS）的纳米粒子，其中麻疹抗原被截留，随后用海藻酸钠包覆。纳米颗粒的尺寸和表面性质可以用不同MW的CS来调节。体外释放研究显示，初始突释，然后延长释放，最适合Makoid-Banakar模型（R-2 > 0.98）。SDS-PAGE分析表明，海藻酸钠包被在酸性条件下能有效保护抗原至少2 h。使用MIT测定法评估HT 29细胞系的细胞活力。将制剂口服给药于小鼠，并使用ELISA方法评价免疫应答。结果表明，麻疹抗原壳聚糖纳米粒诱导了较强的免疫应答，且免疫应答与壳聚糖分子量显著相关。海藻酸钠包被的纳米粒在胃内环境中的抗原保护能力、缓释动力学、全身和粘膜免疫应答以及低细胞毒性表明LMW CS可能是口服疫苗递送的有希望的平台。(C)2014爱思唯尔有限公司版权所有。
In the present study, nanoparticles of low MW chitosan (CS) were formulated in which measles antigen was entrapped and subsequently coated with sodium alginate. The size and surface properties of the nanoparticle can be tuned with different MW of CS. In vitro release studies showed initial burst release followed by extended release, best fitted in the Makoid-Banakar model (R-2 > 0.98). SDS-PAGE assay revealed that alginate coating could effectively protect antigen in acidic condition for at least 2 h. Cell viability was assessed using MIT assay into HT 29 cell line. Formulations were orally administered to mice and immunological responses were evaluated using ELISA method. Obtained results showed that measles antigen-loaded CS nanoparticles induced strong immune response and significant correlation was observed between the immune response with CS MW. Protecting ability of antigen in gastric environment, sustained release kinetics, systemic and mucosal immune responses and low cytotoxicity observed for the alginate coated nanoparticles demonstrated that LMW CS could be promising platform for oral vaccine delivery. (C) 2014 Elsevier Ltd. All rights reserved.