A novel mutant allele at the Cleistogamy 1 locus in barley
A novel mutant allele at the Cleistogamy 1 locus in barley
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DOI：
10.1007/s00122-021-03884-1
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发表时间：
2021-06-14
影响因子：
5.4
通讯作者：
Komatsuda, Takao
中科院分区：
文献类型：
--
作者：
Wang, Ning;Kakeda, Katsuyuki;Komatsuda, Takao
通过将闭花受精的品种暴露于叠氮化钠来诱导裂殖突变体。突变体中改变的cly 1序列不是在miR 172结合位点，就像其他已知的闭花受精等位基因一样，而是在编码基因产物的两个AP 2结构域之一的区域。大麦闭花受精（在花开放之前授粉）的遗传基础集中在闭花受精1位点（cly 1）。属于APETALA 2家族的基因中的microRNA（miR 172）靶向位点的序列在闭花受精和裂花受精品种之间在单个核苷酸位置上不同，导致浆片溶胀的差异能力。在这里，使用叠氮化钠实现的大麦栽培品种“Misato Golden”（携带cly1.b等位基因）的诱变用于诱导从闭花受精到裂花受精（非闭花受精开花）的变化。所选突变体中的cly 1编码序列与cly1.b的编码序列有两个非同义突变，其中一个突变导致Cly 1蛋白中存在的一个AP 2结构域中的残基改变。虽然cly1.b和新等位基因（命名为cly1.b3）之间的miR 172靶向位点没有差异，但突变体的浆片膨胀能力与携带裂殖等位基因Cly1.a的品种中观察到的没有区别。cly1.b3/cly1.b、cly1.b3/cly1.b2和cly1.b3/cly1.c杂合子的表型表明cly1.b3对这些等位基因为隐性或不完全显性。
Key Message A chasmogamous mutant was induced by exposing a cleistogamous cultivar to sodium azide. The altered cly1 sequence in the mutant was not in the miR172 binding site, as is the case in other known cleistogamous alleles, but rather in a region encoding one of the gene product's two AP2 domains. The genetic basis of cleistogamy (in which pollination occurs before the flower opens) in barley is centered on the Cleistogamy 1 locus (cly1). The sequence of the microRNA (miR172)-targeting site in the gene, which belongs to the APETALA2 family, differs between cleistogamous and chasmogamous cultivars at a single nucleotide position, resulting in the differential ability of the lodicules to swell. Here, mutagenesis of the barley cultivar 'Misato Golden' (which carries the cly1.b allele), achieved using sodium azide, was used to induce a change from cleistogamy to chasmogamy (non-cleistogamous flowering). The cly1 coding sequence in the selected mutant differed from that of cly1.b by two non-synonymous mutations, one of which was responsible for an altered residue in one of the AP2 domains present in the Cly1 protein. Although there was no difference in the miR172 targeting site between cly1.b and the novel allele (designated cly1.b3), the mutant's lodicules' ability to swell was indistinguishable from that observed in cultivars carrying the chasmogamous allele Cly1.a. The phenotype of cly1.b3/cly1.b, cly1.b3/cly1.b2 and cly1.b3/cly1.c heterozygotes indicated that cly1.b3 is recessive or incompletely dominant with respect to these alleles.