Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses
Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses
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DOI：
10.1126/science.1062882
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发表时间：
2001-09-07
期刊：
影响因子：
56.9
通讯作者：
Kawaoka, Y
中科院分区：
文献类型：
--
作者：
Hatta, M;Gao, P;Kawaoka, Y
1997年，H5N1甲型流感病毒在香港从鸟类传播给人类，导致18名感染者中的6人死亡。当小鼠感染人类分离株时，两个毒力组变得明显。通过反向遗传学，我们发现 PB2 蛋白 627 位的突变影响了小鼠的感染结果。此外，血凝素糖蛋白的高裂解性是致死感染的基本要求。
In 1997, an H5N1 influenza A virus was transmitted from birds to humans in Hong Kong, killing 6 of the 18 people infected. When mice were infected with the human isolates, two virulence groups became apparent. Using reverse genetics, we showed that a mutation at position 627 in the PB2 protein influenced the outcome of infection in mice. Moreover, high cleavability of the hemagglutinin glycoprotein was an essential requirement for lethal infection.