Detection of Benzene and Alkylated Benzene Derivatives in Fuel Contaminated Environments
Detection of Benzene and Alkylated Benzene Derivatives in Fuel Contaminated Environments
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DOI：
10.1002/clen.201600206
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发表时间：
2016-12-01
影响因子：
1.7
通讯作者：
Levine, Mindy
中科院分区：
文献类型：
--
作者：
DiScenza, Dana J.;Verderame, Molly;Levine, Mindy
本文报道了在2015年3月马萨诸塞州伯恩的伯恩扶轮社发生油罐车撞车事故后，对苯和烷基化苯衍生物的灵敏和选择性检测以及该系统在燃料污染环境检测中的实际应用。这种检测方法依赖于环糊精促进高量子产率荧光团的邻近诱导荧光调制的能力，这导致了所研究的每个环糊精-分析物-荧光团组合的独特的调制响应。使用所测量的荧光发射的变化来生成阵列，该阵列使用线性判别分析来为每个组合生成唯一的图案识别符。该方法成功地检测了分析物的ppm水平，100%成功地区分了纯化缓冲液中的分析物，并100%成功地区分了来自与坠机地点相关的三个采集点的水样中的分析物：下游、上游和直接来自排水管的分析物。值得注意的是，这种方法成功地为二甲苯的结构异构体(邻位、间位、对位)产生了良好分离的信号，如果没有专门的基于质谱学的技术，这是很难做到的。该方法具有高选择性、高灵敏度和广泛的适用性，在检测各种复杂环境中的芳香族化合物方面具有很大的潜力。
Reported herein is the sensitive and selective detection of benzene and alkylated benzene derivatives as well as the practical application of this system in the detection of fuel contaminated environments following a March 2015 tanker truck crash at the Bourne Rotary in Bourne, Massachusetts. This detection method relies on the ability of cyclodextrin to promote proximity-induced fluorescence modulation of a high quantum yield fluorophore, which led to unique modulation responses for each cyclodextrin-analyte-fluorophore combination investigated. The measured changes in fluorescence emission were used to generate arrays using linear discriminant analysis to generate unique pattern identifiers for each combination. The method was successful in detecting ppm levels of analytes with 100% success in differentiating analytes in purified buffer solution and 100% success in differentiating between analytes in water samples from three collection locations related to the crash site: downstream, upstream, and directly from the drainage pipe. Notably, this method was successful in generating well-separated signals for structural isomers of xylene (ortho, meta, para) which is difficult without specialized mass spectrometry-based techniques. The high selectivity, sensitivity, and broad applicability of this method have significant potential in the detection of aromatic analytes in a wide range of complex environments.