Decreased expression of serine protease inhibitor family G1 (SERPING1) in prostate cancer can help distinguish high-risk prostate cancer and predicts malignant progression
Decreased expression of serine protease inhibitor family G1 (SERPING1) in prostate cancer can help distinguish high-risk prostate cancer and predicts malignant progression
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前列腺癌中丝氨酸蛋白酶抑制剂家族G1（SERPING1）表达的降低有助于区分高危前列腺癌并预测恶性进展
DOI：
10.1016/j.urolonc.2018.05.021
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发表时间：
2018-08-01
影响因子：
2.7
通讯作者：
Huang, Hai
中科院分区：
文献类型：
--
作者：
Peng, Shengmeng;Du, Tao;Huang, Hai
目的：探讨前列腺癌(PCa)患者丝氨酸蛋白酶抑制因子家族G1(SERPING1)基因表达下调与预后不良的关系。本研究旨在寻找新的、有效的PCa分子标志物，为PCa的早期筛查、侵袭性PCa的鉴别、预后预测以及减轻PCa的经济负担提供依据。方法：采用免疫组织化学方法检测SERPING1蛋白在前列腺癌和正常前列腺组织中的表达，并结合临床病理参数如Gleason评分、病理分级、临床分期、肿瘤分期、淋巴结转移、远处转移等进行分析。此外，我们使用了癌症基因组图谱(TCGA)数据库、Taylor数据库和Oncomine数据集来验证我们的免疫组织化学结果，并在mRNA水平上研究了SERPING1在PCa中的价值。应用Kaplan-Meier分析和COX回归分析评价SERPING_1与PCa预后的关系。结果：SERPING_1在PCa组织中主要表达于胞浆，在PCa组织中表达较少(P<；0.001)。此外，在我们的组织芯片中，SERPING1SERPING1mRNA的低表达与肿瘤的Gleason评分(P=0.004)、病理分级(P=0.01)和肿瘤的晚期(P=0.005)密切相关。在TCGA数据集和Taylor数据集中，SERPING1低表达与患者年龄越小(TCGA组P=0.02，Taylor组P=0.044)和Gleason评分高(TCGA组P=0.019，P
Purpose: The aim of this study was to investigate the associations of serine proteinase inhibitor family G1 (SERPING1) down-regulation with poor prognosis in patients with prostate cancer (PCa). Furthermore, we aim to find more novel and effective PCa molecular markers to provide an early screening of PCa, distinguish patients with aggressive PCa, predict the prognosis, or reduce the economic burden of PCa.Methods: SERPING1 protein expression in both human PCa and normal prostate tissues was detected by immunohistochemical staining, which intensity was analyzed in association with clinical pathological parameters such Gleason score, pathological grade, clinical stage, tumor stage, lymph node metastasis, and distant metastasis. Moreover, we used The Cancer Genome Atlas (TCGA) Database, Taylor Database, and Oncomine dataset to validate our immunohistochemical results and investigated the value of SERPING1 in PCa at mRNA level. Kaplan-Meier analysis and Cox regression analysis were performed to evaluate the relationship between SERPING1 and prognosis of patients with PCa.Results: The outcome showed that SERPING1 was expressed mainly in cytoplasm of grand cells of prostate tissue and was significantly expressed less in PCa (P < 0.001). Furthermore, in the tissue microarray of our samples, decreasing expression of SERPING1 was correlated with the higher Gleason score (P = 0.004), the higher pathological grade (P = 0.01) and the advanced tumor stage (P = 0.005) at protein level. In TCGA dataset and Taylor Dataset, low-expressed SERPING1 was correlated with the younger patient (P = 0.02 in TCGA, P = 0.044 in Taylor) and the higher Gleason score (P = 0.019 in TCGA, P