Biodegradation and distribution of water-soluble chitosan in mice
Biodegradation and distribution of water-soluble chitosan in mice
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DOI：
10.1016/s0142-9612(98)00159-8
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发表时间：
1999-01-01
期刊：
影响因子：
14
通讯作者：
Machida, Y
中科院分区：
文献类型：
--
作者：
Onishi, H;Machida, Y
随机检测 50% 脱乙酰甲壳素（称为 Chi）对小鼠腹膜内 (ip) 给药后的生物降解性、体内分布和尿排泄情况。使用异硫氰酸荧光素 (FITC) 标记的 Chi (FTC-Chi) 研究了这些特性。通过与溶菌酶和鼠血浆和尿液一起孵育来研究体外生物降解性。溶菌酶、血浆和尿液加速了Chi或FTC-Chi的降解。通过凝胶色谱检查分子量。降解产物显示出相当小的分子量并且不含大分子量的FTC-CM。腹腔注射小鼠后1、14和24小时考察FTC-Chi的体内分布和尿液排泄。 FTC-Chi快速移动至肾脏和尿液，几乎不分布至肝脏、脾脏、腹水和血浆。 14小时后大部分FTC-Chi排泄至尿液中，且排出的FTC-Chi的分子量与体外长时间孵育所得产物的分子量一样小。因此，Chi被认为具有高度的生物降解性，并且很容易从尿液中排出，并且进一步表明在体内积累没有问题；然而，与此同时，人们发现 Chi 不能作为聚合物载体发挥作用，显示出在体内的长期保留。 (C) 1998 Elsevier Science Ltd. 保留所有权利。
Randomly 50% deacetylated chitin, called Chi, was examined on the biodegradability, body distribution and urinary excretion after the intraperitoneal (ip) administration to mice. These characteristics were investigated using fluorescein isothiocyanate (FITC)-labeled Chi (FTC-Chi). The in vitro biodegradability was investigated by incubation with lysozyme and murine plasma and urine. The degradation of Chi or FTC-Chi was accelerated by lysozyme, plasma and urine. The molecular weight was checked by gel-chromatography. The degradation product showed a fairly small molecular weight and contained no FTC-CM of a large one. The body distribution and urinary excretion of FTC-Chi were investigated at 1, 14 and 24 h after the ip injection to mice. FTC-Chi moved fast to the kidney and urine, and was scarcely distributed to the liver, spleen, abdominal dropsy and plasma. Most of FTC-Chi was excreted into urine after 14 h, and the molecular weight of the excreted FTC-Chi was as small as that of the product obtained by the long in vitro incubation. Therefore, Chi is considered to be highly biodegradable and easily excreted in urine, and further it is suggested to have no problem on accumulation in the body; however, at the same time, Chi is found not to operate as a polymer support showing long retention in the body. (C) 1998 Elsevier Science Ltd. All rights reserved.