Autotaxin controls caudal diencephalon-mesencephalon development in the chick
Autotaxin controls caudal diencephalon-mesencephalon development in the chick
复制标题
自分泌运动因子控制雏鸡尾部间脑-中脑的发育
DOI：
10.1002/dvdy.22403
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Aoki J
中科院分区：
文献类型：
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作者：
Ohuchi H;Fukui H;Matsuyo A;Tomonari S;Tanaka M;Arai H;Noji S;Aoki J
间脑是大脑高级整合中心的胚胎原基。最近的研究已经阐明了吻侧间脑细胞如何获得其区域身份。然而，对间脑尾部形成机制的了解仍然有限。在这里，我们专注于自分泌运动因子（ATX）的作用，溶血磷脂生成外切酶，其mRNA在间脑尾部检测。针对ATX的RNA干扰改变了Pax 6调控基因Tcf 4、Lim 1和En 1的表达模式，这意味着ATX是维持间脑尾侧和间脑-中脑边界（DMB）区域同一性所必需的。此外，ATX-RNAi抑制DMB两侧的神经上皮细胞增殖。我们提出了ATX在鸡脑发育中的双重作用，其中ATX不仅作为短程信号促进尾间脑的形成，而且作为长程信号调节中脑的生长。Developmental Dynamics 239：2647-2658，2010.© 2010 Wiley利斯公司
The diencephalon is the embryonic anlagen of the higher integration centers of the brain. Recent studies have elucidated how the cells in the rostral diencephalon acquire their regional identities. However, the understanding of the mechanisms under which the caudal diencephalon is formed is still limited. Here we focus on the role of Autotaxin (ATX), a lysophospholipid‐generating exoenzyme, whose mRNA is detected in the caudal diencephalon. RNA interference againstATXaltered the expression pattern of Pax6‐regualted genes,Tcf4, Lim1, andEn1, implying thatATXis required for the maintenance of the regional identity of the caudal diencephalon and the diencephalon‐mesencephalon boundary (DMB). Furthermore,ATX‐RNAi inhibited neuroepithelial cell proliferation on both sides of the DMB. We propose a dual role of ATX in chick brain development, in which ATX not only contributes to the formation of caudal diencephalon as a short‐range signal, but also regulates the growth of mesencephalon as a long‐range signal. Developmental Dynamics 239:2647–2658, 2010. © 2010 Wiley‐Liss, Inc.