BIOCHEMICAL CHANGES ASSOCIATED WITH DEVELOPMENT AND REVERSAL OF GALACTOSE CATARACTS
BIOCHEMICAL CHANGES ASSOCIATED WITH DEVELOPMENT AND REVERSAL OF GALACTOSE CATARACTS
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DOI：
10.1016/0014-4835(76)90157-3
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发表时间：
1976-01-01
影响因子：
3.4
通讯作者：
LIM, CP
中科院分区：
文献类型：
--
作者：
REDDY, VN;SCHWASS, D;LIM, CP
测定了白内障形成过程中[大鼠]透镜提取物中谷胱甘肽（GSH）的合成速率，结果与正常晶状体提取物相同。与X射线诱导的白内障不同，半乳糖性白内障中GSH合成的潜在机制显然不受影响。测定了半乳糖性白内障发生和逆转过程中GSH、游离氨基酸、半乳糖醇、阳离子和透镜水化程度的变化。即使在成熟白内障发生后（20天），喂食正常饮食也会导致皮质混浊几乎完全消失，留下基本透明的透镜，仅具有非常细的针头状核混浊。在白内障形成期间迅速下降的GSH、牛磺酸和其他游离氨基酸的水平在逆转期恢复到接近正常值，即使晶状体是水合的。相反，这些成分的接近完全恢复，Na+浓度保持4倍以上，几乎4周后，饮食逆转的控制镜头。在同一时期，K+的回收率约为对照的70%。尽管半乳糖醇消失，但在白内障逆转期晶状体的持续水合作用显然与Na+浓度升高有关。对水合晶状体中GSH和游离氨基酸的恢复提出了可能的解释。
The rate of synthesis of glutathione (GSH) in [rat] lens extracts during cataract formation was determined and was the same as that of extracts from normal lenses. Unlike X-ray-induced cataracts the potential mechanism for GSH synthesis in galactose cataracts is apparently unaffected. The levels of GSH, free amino acids, dulcitol, cations and the degree of hydration of the lens during the development and reversal of galactose cataract were determined. Feeding a normal diet even after the development of mature cataracts (20 days) led to the disappearance of cortical opacities almost completely leaving an essentially clear lens with only a very fine pinhead nuclear opacity. The levels of GSH, taurine and other free amino acids, which fell rapidly during cataract formation, returned to near normal values during the reversal phase even though the lenses were hydrated. In contrast to the near complete recovery of these constituents, Na+ concentration remained 4 times higher than in the control lenses almost 4 wk after diet reversal. The recovery of K+ during the same period was about 70% of controls. The continued hydration of the lenses during the reversal phase of cataract, despite the disappearance of dulcitol, was apparently related to the elevated Na+ concentration. A possible explanation for the recovery of GSH and free amino acids in hydrated lenses was suggested.