Immunocytochemical identification of Type I collagen in acid-etched dentin
Immunocytochemical identification of Type I collagen in acid-etched dentin
复制标题
DOI：
10.1002/jbm.a.10073
复制
发表时间：
2003-09-15
影响因子：
4.9
通讯作者：
Lopes, M
中科院分区：
文献类型：
--
作者：
Breschi, L;Perdigao, J;Lopes, M
本研究测试的假设是，根据免疫化学抗原性评估，磷酸的应用会阻止胶原纤维 (CF) 维持其结构形态。牙本质用 EDTA 和 35% H3PO4 处理 15、30 和 60 秒。对于对照，没有进行治疗。在高分辨率 SEM 下评估包埋前免疫组织化学程序，并在 TEM 水平的超薄切片上进行包埋后免疫定位技术。与用 H3PO4 处理的牙本质相比，用 EDTA 处理产生的标记信号更弱。在用 H3PO4 蚀刻牙本质 15 秒后观察到最强烈的标记。当施加酸 30 秒或 60 秒时，标记强度最低。在未经处理的牙本质表面上检测到非常微弱的信号。这些结果证明，应用 15 秒的 35% H3PO4 会导致包裹表面 CF 的晶体发生矿物质溶解，而不会损坏 CF 的超微结构，而较长时间应用 35% H3PO4 会导致 CF 发生变化，从而降低其抗原性。 (C) 2003 年 Wiley 期刊公司。
The hypothesis tested in this study is that the application of phosphoric acid prevents collagen fibrils (CF) from maintaining their structural morphology, as assessed by their immunochemical antigenicity. Dentin was conditioned with EDTA and with 35% H3PO4 for 15, 30, and 60 s. For a control there was no treatment. A pre-embedding immunohistochemical procedure was evaluated under high-resolution SEM and a postembedding immunolocalization technique was performed on ultrathin sections at TEM level. Conditioning with EDTA resulted in a weaker labeling signal than for dentin conditioned with H3PO4. The most intense labeling was observed after dentin had been etched with H3PO4 for 15 s. The least intensive labeling was evident when the acid was applied for 30 s or for 60 s. A very weak signal was detected on the untreated dentin surface. These results provide evidence that a 15-s application of 35% H3PO4 causes mineral dissolution of the crystals enveloping the superficial CF without damaging the ultrastructure of the CF while longer applications of 35% H3PO4 cause alterations in the CF that decrease their anti genicity. (C) 2003 Wiley Periodicals, Inc.