Force Transduction by the Microtubule-Bound Dam1 Ring
Force Transduction by the Microtubule-Bound Dam1 Ring
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DOI：
10.1016/j.bpj.2010.01.004
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发表时间：
2010-04-21
影响因子：
3.4
通讯作者：
Turner, Matthew S.
中科院分区：
文献类型：
--
作者：
Armond, Jonathan W.;Turner, Matthew S.
微管（MT）的解聚和Dam1环复合物的运动之间的耦合现在被认为在有丝分裂期间力的产生中起重要作用。我们目前对这种运动的理解是基于一些详细的计算模型。虽然这些模型实现了可能的机制，力的转换，他们可以扩展的变化的任何大量的测量不佳的参数，并没有明确的策略，以确定如何区分它们可能是实验。在这里，我们试图识别和分析两个不同的机制存在于计算模型。在第一种情况下，解聚的MT末端的张开的原丝在物理上防止Dam1环从末端脱落，而在另一种情况下，吸引力结合将环固定到微管上。基于这种分析，我们讨论了如何区分竞争模型，试图解释如何Dam1环留在MT。我们提出了新的实验方法，可以解决这些模型的第一次，无论是通过改变扩散常数的Dam1环（例如，通过将长的聚合物系在其上）或通过使用随时间变化的负载。
The coupling between the depolymerization of microtubules (MTs) and the motion of the Dam1 ring complex is now thought to play an important role in the generation of forces during mitosis. Our current understanding of this motion is based on a number of detailed computational models. Although these models realize possible mechanisms for force transduction, they can be extended by variation of any of a large number of poorly measured parameters and there is no clear strategy for determining how they might be distinguished experimentally. Here we seek to identify and analyze two distinct mechanisms present in the computational models. In the first, the splayed protofilaments at the end of the depolymerizing MT physically prevent the Dam1 ring from falling off the end, and in the other, an attractive binding secures the ring to the microtubule. Based on this analysis, we discuss how to distinguish between competing models that seek to explain how the Dam1 ring stays on the MT. We propose novel experimental approaches that could resolve these models for the first time, either by changing the diffusion constant of the Dam1 ring (e.g., by tethering a long polymer to it) or by using a time-varying load.