Signal amplification and detection via a supramolecular allosteric catalyst
Signal amplification and detection via a supramolecular allosteric catalyst
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DOI：
10.1021/ja0437306
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发表时间：
2005-02-16
影响因子：
15
通讯作者：
Mirkin, CA
中科院分区：
文献类型：
--
作者：
Gianneschi, NC;Nguyen, ST;Mirkin, CA
设计了一种用于催化信号放大和检测的超分子变构催化剂体系。催化剂通过引入也表现为变构活化剂的分析物而被“打开”。低至800 nM的Cl-离子浓度进行催化扩增和检测。通过pH敏感的荧光探针转导信号，并使用实验室手持UV灯和分光光度法进行目视观察。此外，使用气相色谱法对一系列Cl-浓度的变构效应进行定量。这三部分检测方案涉及分析物结合，变构催化剂活化，和信号转导代表了一种新的方法来小分子检测。
The design of a supramolecular allosteric catalyst system for catalytic signal amplification and detection is presented. The catalyst was switched “on” by the introduction of an analyte that also behaves as an allosteric activator. Concentrations of Cl-ions as low as 800 nM were catalytically amplified and detected. The signal was transduced via a pH-sensitive fluorescent probe and observed visually using a laboratory, handheld UV lamp and by spectrophotometry. Furthermore, the allosteric effect was quantified using gas chromatography for a range of Cl-concentrations. This three-part detection scheme involving analyte binding, allosteric catalyst activation, and signal transduction represents a new approach to small-molecule detection.