LONG-TERM HUMAN B-CELL LINES DEPENDENT ON INTERLEUKIN-4 AND ANTIBODY TO CD40
LONG-TERM HUMAN B-CELL LINES DEPENDENT ON INTERLEUKIN-4 AND ANTIBODY TO CD40
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DOI：
10.1126/science.1702555
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发表时间：
1991-01-04
期刊：
影响因子：
56.9
通讯作者：
ROUSSET, F
中科院分区：
文献类型：
--
作者：
BANCHEREAU, J;DEPAOLI, P;ROUSSET, F
CD 40是一种45- 50千道尔顿的跨膜糖蛋白，在B淋巴细胞、上皮细胞和一些癌细胞系上表达。 人静息B淋巴细胞在与已用人Fc受体（Fc-γ-RII/CDw 32）和抗CD 40单克隆抗体转染的小鼠成纤维细胞Ltk-细胞系孵育时进入持续增殖状态。 与白细胞介素-4组合，产生了对EB病毒感染始终呈阴性的因子依赖性长期正常人B细胞系。 因此，CD 40的交联可能代表B细胞克隆扩增中的重要现象。
CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk- cell line that had been transfected with the human Fc receptor (Fc-gamma-RII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.