The effects of Ankrd2 alteration indicate its involvement in cell cycle regulation during muscle differentiation
The effects of Ankrd2 alteration indicate its involvement in cell cycle regulation during muscle differentiation
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DOI：
10.1016/j.bbamcr.2008.01.027
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发表时间：
2008-06-01
影响因子：
5.1
通讯作者：
Lanfranchi, Gerolamo
中科院分区：
文献类型：
--
作者：
Bean, Camilla;Facchinello, Nicola;Lanfranchi, Gerolamo
Ankrd2是肌肉锚蛋白重复蛋白家族（MARP）的成员，由也可以定位于细胞核的肌节相关蛋白组成。有迹象表明，MARP可能作为细胞质和细胞核之间的穿梭蛋白发挥作用，可能向细胞核发送有关收缩机制结构或功能变化的信息。尽管最近的研究结果表明，MARP基因家族是不是必不可少的骨骼肌的基础功能，其对骨骼肌细胞的基因表达程序的影响被强调。为了研究这种调节作用，我们产生并检查了稳定过表达或沉默Ankrd2蛋白的心肌细胞的形态和功能特征。肌细胞的转录谱显示，Ankrd2蛋白水平的变化所干扰的分子途径与Ankrd2修饰的诱导分化的成肌细胞中获得的形态生理和生化数据一致。我们的研究结果表明，Ankrd2提供了一个重要的贡献，在肌细胞分化过程中的协调增殖和凋亡在体外，主要是通过p53网络。(C)2008 Elsevier B.V.保留所有权利。
Ankrd2 is a member of the Muscle Ankyrin Repeat Protein family (MARPs), consisting of sarcomere-associated proteins that can also localize in the nucleus. There are indications that MARPs might function as shuttle proteins between the cytoplasm and nucleus, likely sending information to the nucleus concerning the changes in the structure or function of the contractile machinery. Even though recent findings suggest that the MARP gene family is not essential for the basal functioning of skeletal muscle, its influence on the gene expression program of skeletal muscle cells was highlighted. To investigate this regulatory role we produced and examined both morphological and functional features of myocytes stable overexpressing or silencing the Ankrd2 protein. The transcriptional profiles of the myocytes revealed that the molecular pathways perturbed by changes in Ankrd2 protein level are congruent with the morpho-physiological and biochemical data obtained in Ankrd2-modified myoblasts induced to differentiate. Our results suggest that Ankrd2 gives an important contribution to the coordination of proliferation and apoptosis during myogenic differentiation in vitro, mainly through the p53 network. (C) 2008 Elsevier B.V. All rights reserved.