LET-dependent effects of heavy-ion beam irradiation in Arabidopsis thaliana
LET-dependent effects of heavy-ion beam irradiation in Arabidopsis thaliana
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DOI：
10.5511/plantbiotechnology.25.113
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Abe, Tomoko
中科院分区：
文献类型：
--
作者：
Kazama, Yusuke;Saito, Hiroyuki;Abe, Tomoko
采用几种重离子束辐照拟南芥，研究重离子束辐照的线性能量传递效应。首先，用C、N、Ne、Ar或Fe离子以5至400戈伊的剂量照射干种子，以比较离子种类之间的开花率和突变率。开花和突变率对辐照的敏感性在离子种类之间有显著差异。在离子种类中，N（30 keV μ m（-1））在诱导白化病植物方面是最有效的。其次，我们研究了LET对突变诱导的影响。C、N、Ne和Ar离子束的LET控制在30-640 keV μ m（-1）。无论离子种类如何，相同LET值的辐照导致相同的开花率和突变率。因此，离子束的LET似乎是影响诱变的一个重要因素。我们发现了一个440 bp的缺失hy（延长下胚轴）的突变体，是从M-2后代分离。这些关于LET依赖性的基本数据可用于开发植物诱变的先进技术。
We irradiated Arabidopsis thaliana with several kinds of heavy-ion beams to investigate the linear energy transfer (LET)-dependent effects of heavy-ion beam irradiation. First, dry seeds were irradiated with C, N, Ne, Ar, or Fe ions at doses ranging from 5 to 400 Gy to compare the flowering and mutation rates among the ion species. The sensitivity of the flowering and mutation rates to irradiation differed markedly among the ion species. Of the ion species, N (30 keV mu m(-1)) was the most effective at inducing albino plants. Second, we examined the effects of LET on mutation induction. The LET of C, N, Ne, and Ar ion beams was controlled at 30-640 keV mu m(-1). Regardless of ion species, irradiation with the same LET value resulted in the same flowering rate and mutation rate. Thus, the LET of ion beams seems to be an important factor affecting mutagenesis. We found a 440-bp deletion in the hy (elongated hypocotyl) mutant that was isolated from M-2 progeny. These fundamental data on LET-dependence can be used to develop advanced technologies for plant mutagenesis.