tRF-Leu-CAG promotes cell proliferation and cell cycle in non-small cell lung cancer.
tRF-Leu-CAG promotes cell proliferation and cell cycle in non-small cell lung cancer.
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DOI：
10.1111/cbdd.12994
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发表时间：
2017-11
影响因子：
3
通讯作者：
Ma Z
中科院分区：
文献类型：
--
作者：
Shao Y;Sun Q;Liu X;Wang P;Wu R;Ma Z
tRNA衍生的RNA片段（trf）是长度为14 - 35nt的非编码单链RNA，在基因调控中起着重要作用，甚至在致癌过程中也起着重要作用。在这项研究中，我们研究了tRF - Leu - CAG在人非小细胞肺癌（NSCLC）中的表达及其在NSCLC细胞增殖和细胞周期中的作用。在NSCLC组织、细胞系和血清中检测tRF - Leu - CAG的表达水平。非小细胞肺癌肿瘤组织中tRF - Leu - CAG RNA水平高于正常组织，并且在非小细胞肺癌细胞系中也上调。分期进展与非小细胞肺癌血清中tRF - Leu - CAG之间存在显著关系。我们发现，在H1299细胞中，抑制tRF - Leu - CAG抑制了细胞增殖，阻碍了细胞周期。AURKA也因tRF - Leu - CAG的下调而受到抑制。因此，我们的研究表明tRF - Leu - CAG可能参与AURKA的调控，可能成为NSCLC新的诊断标志物和潜在的治疗靶点。
tRNA‐derived RNA fragments (tRFs), non‐coding single‐stranded RNAs with 14–35 nt in length, were found to play important roles in gene regulation, even in carcinogenesis. In this study, we investigated the expression of tRF‐Leu‐CAG in human non‐small cell lung cancer (NSCLC) and its function in the cell proliferation and cell cycle of NSCLC. The expression level of tRF‐Leu‐CAG was detected in NSCLC tissues, cell lines, and sera. tRF‐Leu‐CAG RNA levels were higher in NSCLC tumor tissues than in normal tissues, and also upregulated in NSCLC cell lines. A significant relationship was observed between stage progression and tRF‐Leu‐CAG in NSCLC sera. We found that in H1299 cells, inhibition of tRF‐Leu‐CAG suppressed cell proliferation and impeded cell cycle. AURKA was also repressed with the knockdown of tRF‐Leu‐CAG. Thus, our study revealed that tRF‐Leu‐CAG may be involved in regulating AURKA and could be a new diagnostic marker and potential therapeutic target in NSCLC.