Calmodulin: localization in plant tissues.
Calmodulin: localization in plant tissues.
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DOI：
10.1177/34.5.3084624
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发表时间：
1986-05
期刊：
影响因子：
--
通讯作者：
C T Lin;D. Sun;G X Song;J Y Wu
中科院分区：
文献类型：
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作者：
C T Lin;D. Sun;G X Song;J Y Wu
通过制备型 SDS-聚丙烯酰胺凝胶电泳从牛脑中纯化钙调蛋白。变性的纯化钙调蛋白用于免疫兔子以产生抗血清。该抗血清用于通过间接免疫组织化学研究钙调蛋白在植物组织中的分布。研究人员对玉米种子、燕麦种子、花生、意大利面南瓜种子和菠菜顶芽的根尖进行了研究。开发了一种植物组织切片和抑制内源过氧化物活性的方法。在玉米根部切片中，抗钙调蛋白的反应产物主要存在于根冠细胞中。较少但显着量的钙调蛋白位于后木质部元件、后木质部元件周围的一些石柱细胞、顶端首字母和皮质细胞中。在燕麦种子、花生和意大利面南瓜种子的其他根尖中也观察到了类似的发现。在菠菜的顶芽中，钙调蛋白染色的细胞高度集中在顶端分生组织和叶原基中。这些发现表明根冠中高浓度的钙调蛋白可能对向地性和生长发育很重要。
Calmodulin was purified from bovine brain by preparative SDS-polyacrylamide gel electrophoresis. The denatured, purified calmodulin was used to immunize rabbits to produce antiserum. This antiserum was used to study the distribution of calmodulin in plant tissues by indirect immunohistochemistry. The root tips from corn seeds, oat seeds, peanuts, spaghetti squash seeds, and the terminal buds of spinach were investigated. A method for plant tissue sectioning and inhibition of endogenous peroxide activity was developed. In the corn root section, reaction product from anti-calmodulin was found mainly in the root cap cells. Lesser but significant amounts of calmodulin were localized in metaxylem elements, in some stele cells surrounding metaxylem elements, in apical initials, and in the cortical cells. Similar findings were also observed in other root tips from oat seeds, peanuts, and spaghetti squash seeds. In the terminal buds of the spinach, calmodulin-stained cells were highly concentrated in the apical meristem and leaf primordium. These findings suggest that the high concentration of calmodulin in the root cap may be important in relation to gravitropism and growth development.