GERM-LINE TRANSMISSION OF A C-ABL MUTATION PRODUCED BY TARGETED GENE DISRUPTION IN ES CELLS
GERM-LINE TRANSMISSION OF A C-ABL MUTATION PRODUCED BY TARGETED GENE DISRUPTION IN ES CELLS
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DOI：
10.1126/science.2554496
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发表时间：
1989-11-10
期刊：
影响因子：
56.9
通讯作者：
ROBERTSON, EJ
中科院分区：
文献类型：
--
作者：
SCHWARTZBERG, PL;GOFF, SP;ROBERTSON, EJ
通过外源添加的DNA与内源基因的同源重组，在小鼠胚胎干细胞的c-abl基因座引入了替代突变，并将这些细胞用于产生嵌合体小鼠。结果表明，c-abl突变是通过多个雄性嵌合体传递给后代的。这项工作证明了通过同源重组将突变引入到非选择性常染色体基因中进行种系传递的可行性。
A substitution mutation has been introduced into the c-abl locus of murine embryonic stem cells by homologous recombination between exogenously added DNA and the endogenous gene, and these cells have been used to generate chimetric mice. It is shown that the c-abl mutation was transmitted to progeny by several male chimeras. This work demonstrates the feasibility of germ-line transmission of a mutation introduced into a nonselectable autosomal gene by homologous recombination.