Temporal response of murine pluripotent stem cells and myeloid and erythroid progenitor cells to low-dose glucan treatment.
Temporal response of murine pluripotent stem cells and myeloid and erythroid progenitor cells to low-dose glucan treatment.
复制标题
小鼠多能干细胞以及骨髓和红系祖细胞对低剂量葡聚糖治疗的时间反应。
作者：
M. Patchen;T. MacVittie
B6D2F1雌性小鼠静脉注射葡聚糖0.4 mg。分别于1、5、11、17d后检测骨髓和脾细胞总数、多能造血干细胞(CFU-S)、粒-巨噬祖细胞(GM-CFCs)、红系集落形成细胞(CFU-e)和爆裂形成细胞(BFU-e)的数量。骨髓TNC没有改变，但脾TNC在第5天增加了近两倍，并在葡聚糖治疗后第11天和第17天保持增加。骨髓和脾CFU-E浓度在给药后第5天显著升高(P<0.01)，第17天恢复到对照水平。脾CFU-e浓度在给药后第5、11、17天升高，而BFU-e浓度仅在给药后第11天升高。相比之下，经葡聚糖治疗后，骨髓CFU-e和BFU-e浓度不受影响或略有下降。当检测外周血中CFU-S和GM-CFCs时，在葡聚糖治疗后的任何时候，这些祖细胞的浓度都没有检测到明显的增加。低剂量(0.4毫克)葡聚糖治疗的这些效应与先前报道的高剂量(例如4.0毫克)葡聚糖治疗的效应之间的相关性进行了讨论。
B6D2F1 female mice were intravenously administered 0.4 mg of glucan. 1, 5, 11, and 17 days later, the total nucleated cellularity (TNC) and the numbers of pluripotent hemopoietic stem cells (CFU-s), granulocyte-macrophage progenitor cells (GM-CFC), and erythroid colony-forming (CFU-e) and burst-forming (BFU-e) cells were assayed in the bone marrow and spleen. Bone marrow TNC was not altered, but splenic TNC increased approximately twofold on day 5 and remained increased on days 11 and 17 after glucan treatment. The concentrations of bone marrow and splenic CFU-s and GM-CFC both significantly increased (p less than 0.01) by 5 days after glucan administration; however, they returned to control levels by day 17. Splenic CFU-e concentration increased on days 5, 11, and 17, whereas splenic BFU-e concentration increased only on day 11 after treatment. By contrast, bone marrow CFU-e and BFU-e concentrations were either unaffected or slightly decreased by glucan treatment. When peripheral blood was assayed for CFU-s and GM-CFC, no detectable increase in the concentrations of these progenitors was noted at any time after glucan treatment. The relevance of these effects of low-dose (0.4 mg) glucan treatment is discussed with respect to previously reported effects of higher-dose (e.g., 4.0 mg) glucan treatment.