Forward mandibular positioning enhances the expression of Ang-1 and Ang-2 in rabbit condylar chondrocytes
Forward mandibular positioning enhances the expression of Ang-1 and Ang-2 in rabbit condylar chondrocytes
复制标题
下颌前倾定位增强兔髁突软骨细胞中 Ang-1 和 Ang-2 的表达。
DOI：
10.3892/mmr.2013.1620
复制
发表时间：
2013-10-01
影响因子：
3.4
通讯作者：
Feng, Jianying
中科院分区：
文献类型：
--
作者：
Jing, Zhan;Gu, Zhiyuan;Feng, Jianying
功能矫治器矫正牙错，部分是通过对髁突软骨施加间接的机械刺激，在髁突中启动新的骨形成。血管生成素参与与新骨形成相关的血管生成。本研究旨在检测兔下颌前定位（FMP）后血管生成素(Ang) - 1和- 2在髁突软骨细胞中的表达。将60只8周龄的家兔随机分为试验组和对照组，每组30只。实验组采用功能性器具诱导FMP。实验组和对照组分别于第3天和第1、2、4、8、12周处死5只。采集右侧颞下颌关节（TMJs），免疫组化染色检测Ang - 1和-2的表达。Ang-1的表达在第3天增加，在第2周达到峰值，而Ang- 2在FMP后第4周达到最大表达。随后，Ang - 1和Ang - 2的表达逐渐降低。因此，FMP增强了髁突软骨中Ang - 1和Ang - 2的表达，这与软骨内成骨过程中的血管生成有关。
Functional appliances correct dental malocclusion, partly by exerting an indirect mechanical stimulus on the condylar cartilage, initiating novel bone formation in the condyle. Angiopoietin is involved in the angiogenesis associated with novel bone formation. This study aimed to determine the expression of angiopoietin (Ang)‑1 and ‑2 following forward mandibular positioning (FMP) in the condylar chondrocytes of rabbits. Sixty rabbits (age, 8 weeks) were randomly allocated to the experimental and control groups (n=30 per group). In the experimental group, FMP was induced by a functional appliance. Five rabbits from the experimental group and the control group were sacrificed following 3 days and 1, 2, 4, 8 and 12 weeks, respectively. The right temporomandibular joints (TMJs) were collected and the expression of Ang‑1 and -2 was evaluated by immunohistochemical staining. The expression of Ang-1 increased at day 3 and reached a peak at 2 weeks, whereas Ang‑2 reached maximal expression 4 weeks after FMP. Subsequently, the expression of Ang‑1 and ‑2 gradually decreased. Thus, FMP enhanced the expression of Ang‑1 and Ang‑2 in condylar cartilage, which is related to angiogenesis in the process of endochondral ossification.