MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map
MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map
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DOI：
10.1093/nar/gkr490
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发表时间：
2011-07-01
影响因子：
14.9
通讯作者：
Sali, Andrej
中科院分区：
文献类型：
--
作者：
Tjioe, Elina;Lasker, Keren;Sali, Andrej
电子显微镜(EM)的进步使人们能够以越来越高的分辨率确定大型生物组件的结构。这一过程中的一个关键步骤是将多个组件结构适配到它们组装的EM导出的密度图中。在这里，我们描述用于此任务的Web服务器。服务器接受PDB格式的一组蛋白质结构和MRC格式的EM密度图作为输入。输出是按模型与密度贴图的拟合质量进行排序的模型集合。这些模特可以在线查看，也可以从网站下载。这项服务的网址是：http://salilab.org/multifit/和http://bioinfo3d.cs.tau.ac.il/.
Advances in electron microscopy (EM) allow for structure determination of large biological assemblies at increasingly higher resolutions. A key step in this process is fitting multiple component structures into an EM-derived density map of their assembly. Here, we describe a web server for this task. The server takes as input a set of protein structures in the PDB format and an EM density map in the MRC format. The output is an ensemble of models ranked by their quality of fit to the density map. The models can be viewed online or downloaded from the website. The service is available at; http://salilab.org/multifit/ and http://bioinfo3d.cs.tau.ac.il/.