The effects of PEG-based surface modification of PDMS microchannels on long-term hemocompatibility
The effects of PEG-based surface modification of PDMS microchannels on long-term hemocompatibility
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DOI：
10.1002/jbm.a.35090
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发表时间：
2014-12-01
影响因子：
4.9
通讯作者：
Potkay, Joseph A.
中科院分区：
文献类型：
--
作者：
Kovach, Kyle M.;Capadona, Jeffrey R.;Potkay, Joseph A.
目前的研究表明，聚二甲基硅氧烷（PDMS）微流体网络的第一个表面改性，显示了较长的保质期以及延长的血液相容性。未涂覆的PDMS微通道网络在血液接触应用中快速吸附高水平的纤维蛋白原。纤维蛋白原吸附引发血小板活化，并导致跨微通道网络的压力快速增加，使其无法用于长期应用。在这里，我们描述了使用氧等离子体预处理和聚（乙二醇）接枝的方法密封的PDMS微通道的修改。我们目前的测试结果涂层微通道后，延长老化和血液暴露。我们的PEG接枝通道在应用后长达28天显示出显著降低的纤维蛋白原吸附和血小板粘附，突出了涂层随时间的稳定性和功能性。我们的涂层微通道网络也显示出在全血流动下凝血反应的显著降低。此外，通过涂覆的微通道网络的压力比未涂覆的对照增加一倍所需的时间长16倍以上。总的来说，我们的数据意味着在许多血液接触应用中微流体装置的涂层平台的潜力。(c)2014 Wiley Periodicals，Inc. J Biomed Mater Res Part A：102A：4195-4205，2014.
The current study demonstrates the first surface modification for poly(dimethylsiloxane) (PDMS) microfluidic networks that displays a long shelf life as well as extended hemocompatibility. Uncoated PDMS microchannel networks rapidly adsorb high levels of fibrinogen in blood contacting applications. Fibrinogen adsorption initiates platelet activation, and causes a rapid increase in pressure across microchannel networks, rendering them useless for long term applications. Here, we describe the modification of sealed PDMS microchannels using an oxygen plasma pretreatment and poly(ethylene glycol) grafting approach. We present results regarding the testing of the coated microchannels after extended periods of aging and blood exposure. Our PEG-grafted channels showed significantly reduced fibrinogen adsorption and platelet adhesion up to 28 days after application, highlighting the stability and functionality of the coating over time. Our coated microchannel networks also displayed a significant reduction in the coagulation response under whole blood flow. Further, pressure across coated microchannel networks took over 16 times longer to double than the uncoated controls. Collectively, our data implies the potential for a coating platform for microfluidic devices in many blood-contacting applications. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4195-4205, 2014.