SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids.
SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids.
复制标题
SERS 微传感器，用于测量干细胞衍生的人体气道类器官的管腔和 ECM 中的 pH 值。
DOI：
10.1039/d2cc06582g
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Skinner WH
中科院分区：
文献类型：
--
作者：
Skinner WH
患者来源的类器官具有改善体外疾病模型的生理相关性的潜力。然而，这些自组装细胞结构的3D结构使得探测它们的生物化学比传统的2D培养更加复杂。我们探索应用表面增强拉曼散射微传感器（SERS-MS）探测局部pH梯度内患者来源的气道类器官培养。SERS-MS由用表面固定的金纳米颗粒修饰的固体聚合物核组成，所述金纳米颗粒用pH敏感的报告分子4-巯基苯甲酸（MBA）官能化。我们证明，通过将SERS-MS混合到气道类器官培养物的细胞外基质（ECM）中，探针可以被扩展的类器官吞噬，并报告类器官腔和ECM中的局部pH。
Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.