Loss of Cirbp expression is correlated with the malignant progression and poor prognosis in nasopharyngeal carcinoma
Loss of Cirbp expression is correlated with the malignant progression and poor prognosis in nasopharyngeal carcinoma
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Cirbp表达缺失与鼻咽癌恶性进展和不良预后相关
DOI：
10.2147/cmar.s211389
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发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Xiao, Dong
中科院分区：
文献类型：
--
作者：
Lin, Tao-Yan;Chen, Yan;Xiao, Dong
目的：探讨冷诱导rna结合蛋白（Cirbp）表达与鼻咽癌患者临床病理特征及预后的关系。方法：采用qRT-PCR或免疫组化（IHC）检测Cirbp在鼻咽癌细胞株和组织标本中的表达。结果：免疫组化（IHC）结果显示，61例非癌性鼻咽鳞状上皮组织中有61例Cirbp高表达，而鼻咽癌组织中Cirbp表达明显降低。此外，Cirbp蛋白的免疫组化检测结果显示，177例鼻咽癌和鼻咽癌鳞状上皮细胞的胞核信号较强，细胞质信号较弱，而在许多其他肿瘤中，Cirbp蛋白主要存在于细胞质中。更重要的是，TNM分类显示，与T1-T2、N0-N1、M0和I-II肿瘤以及分化的非角化癌（DNKC）相比，Cirbp的低表达在T3-T4、N2-N3、M1和III-IV型鼻咽癌和未分化癌（UDC）中更为常见，这表明Cirbp的缺失是晚期鼻咽癌的关键分子事件。Kaplan-Meier生存分析表明，Cirbp低表达的NPC患者的总生存时间明显短于Cirbp高表达的NPC患者。多因素分析表明，Cirbp表达水平是鼻咽癌生存的独立预后指标。最后，我们发现在鼻咽癌活检中，Cirbp表达与E-cadherin呈显著正相关，而Cirbp表达与Ki67标记指数呈显著负相关。结论：综上所述，这些发现表明Cirbp表达缺失与鼻咽癌的恶性进展和不良预后相关。
Purpose: The correlation of cold-inducible RNA-binding protein (Cirbp) expression with clinicopathological features including patient prognosis in nasopharyngeal carcinoma (NPC) was investigated. Methods: The expression of Cirbp in NPC cell lines and tissue specimens was examined by qRT-PCR or immunohistochemistry (IHC). Results: Immunohistochemistry (IHC) results showed that high Cirbp expression was detected in 61 of 61 non-cancerous nasopharyngeal squamous epithelial biopsies, whereas the significantly reduced expression of Cirbp was observed in NPC specimens. In addition, IHC assay for Cirbp protein illustrated that the cells of 177 NPC samples and nasopharyngeal squamous epithlial cells displayed strong signals in nuclei and faint signals in cytoplasm, whereas Cirbp protein is mainly detected in the cell’s cytoplasm in many other cancers. More importantly, TNM classification displayed that the low expression of Cirbp was more frequently observed in T3-T4, N2-N3, M1 and III-IV NPC biopsies, and undifferentiated carcinoma (UDC) than T1-T2, N0-N1, M0 and I-II tumors, and differentiated nonkeratinizing carcinoma (DNKC), suggesting that Cirbp loss is a key molecular event in advanced cases of NPC. Kaplan–Meier survival analysis indicated that NPC patients showing lower Cirbp expression had a significantly shorter overall survival time than those with high Cirbp expression. Multivariate analysis suggested that the level of Cirbp expression was an independent prognostic indicator for NPC survival. Finally, we revealed a significant positive association between Cirbp expression and E-cadherin, and a notable negative correlation between Cirbp expression and Ki67 labeling index in NPC biopsies. Conclusion: Collectively, these findings demonstrate that loss of Cirbp expression is correlated with malignant progression and poor prognosis in NPC.