Behavioural characterization of interleukin-6 overexpressing or deficient mice during agonistic encounters
Behavioural characterization of interleukin-6 overexpressing or deficient mice during agonistic encounters
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DOI：
10.1046/j.1460-9568.1998.00377.x
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发表时间：
1998-12-01
影响因子：
3.4
通讯作者：
Panerai, AE
中科院分区：
文献类型：
--
作者：
Alleva, E;Cirulli, F;Panerai, AE
白细胞介素8(IL-6)是一种由激活的免疫细胞释放的影响脑功能的细胞因子，本实验研究了中枢神经系统不表达(IL-6-/-)或过度表达(NSE-HLL-6)IL-6的转基因雄性小鼠在激进化中表现出的攻击性和从属行为。所有受试者在遭遇攻击性之前都被隔离了24天，在测试时已经52天大了。受试者被放置在中性笼子里连续5天，与之前被隔离相同时间的Balb/c株的对手15分钟，第一次和最后一次测试过程被录像，以分别评估第一种方法和社会角色的建立。随后对许多行为类别进行了评分，与野生型对照组相比，IL-6-/-小鼠表现出更高程度的攻击性行为，表现为攻击性直立姿势的频率更高，这种影响在第五次遭遇时更加明显。相反，NSE-HLL-6受试者表现出更多地参与从属类型的社交互动的趋势，表现出更高的频率和持续时间的行为，如肛门、鼻子或身体嗅觉。与对照组相比，IL-6-/-小鼠表现出明显的较少的从属相互作用，而这些小鼠的一些大脑区域的多巴胺水平被发现发生了改变。总体而言，这些数据表明，IL-6影响攻击性和从属类型的相互作用，尽管NSE-HLL-6受试者的行为受到的影响小于IL-6-/-组。还讨论了动物的遗传背景在筛选基因操作结果对行为的影响。
Interleukin-8 (IL-6) is a cytokine released by activated immune cells which has been shown to affect brain function, In this experiment aggressive and affiliative behaviour exhibited during agonistic encounters by transgenic male mice either not expressing (IL-6 -/-) or overexpressing (NSE-hlL-6) IL-6 in the central nervous system was investigated. All subjects were isolated for 24 days before the aggressive encounter and were 52 days old at the time of testing. Subjects were placed for 5 consecutive days in a neutral cage for 15 min with an opponent of the Balb/c strain that had been previously isolated for the same amount of time, The first and the last test sessions were videotaped to evaluate the first approach and the establishment of the social role, respectively. A number of behavioural categories were later scored, When compared with wild-type controls, IL-6 -/- mice showed a higher degree of aggressive behaviour as indicated by a higher frequency of Offensive Upright Posture, an effect more pronounced on the fifth encounter. On the contrary, NSE-hlL-6 subjects showed a tendency to be more involved in affiliative-type social interactions, displaying a higher frequency and duration of behaviours such as Anogenital, Nose or Body Sniff. IL-6 -/- mice showed a clear tendency to exhibit less affiliative interactions compared with their controls while dopamine levels were found to be modified in a number of brain regions in these mice.Overall, these data suggest that IL-6 affects both aggressive and affiliative-type interactions, although the behaviour of the NSE-hlL-6 subjects was less affected than that of the IL-6 -/- group. The effects of the genetic background of the animal in screening the outcome of gene manipulations on behaviour are also discussed.