Development of Molecularly Imprinted Polymers to Target Polyphenols Present in Plant Extracts
Development of Molecularly Imprinted Polymers to Target Polyphenols Present in Plant Extracts
复制标题
DOI：
10.3390/pr5040072
复制
发表时间：
2017-12-01
期刊：
影响因子：
3.5
通讯作者：
Costa, Mario Rui P. F. N.
中科院分区：
文献类型：
--
作者：
Gomes, Catarina;Sadoyan, Gayane;Costa, Mario Rui P. F. N.
针对蔬菜提取物中的多酚类化合物，发展了分子印迹聚合物。白藜芦醇苷由于其相对较大的尺寸和两亲性而被选择作为模板多酚。合成了不同的分子印迹聚合物，以探索功能单体和模板分子之间的优先相互作用。溶剂极性对分子印迹效率的影响，即由于疏水相互作用，也进行了评估。沉淀和悬浮聚合作为一种可能的方式来改变分子印迹聚合物的形态和性能进行了研究。固相萃取和批量/连续吸附过程中使用的多酚的吸收/释放，在个人/竞争性试验进行评估。其中以4-乙烯基吡啶为功能单体，水/甲醇为溶剂的悬浮聚合法合成的分子印迹聚合物表现出了较好的上级性能。这种显著结果的根本原因是由虎杖苷的两亲性质引起的可能的表面印迹过程。天然提取物中存在的多酚的吸收和随后的选择性释放被成功地证明，考虑红葡萄酒溶液作为案例研究。然而，亲水/疏水相互作用是不可避免的（特别是复杂的天然提取物），溶剂极性的调整是分离不同多酚的重要问题。
The development of molecularly imprinted polymers (MIPs) to target polyphenols present in vegetable extracts was here addressed. Polydatin was selected as a template polyphenol due to its relatively high size and amphiphilic character. Different MIPs were synthesized to explore preferential interactions between the functional monomers and the template molecule. The effect of solvent polarity on the molecular imprinting efficiency, namely owing to hydrophobic interactions, was also assessed. Precipitation and suspension polymerization were examined as a possible way to change MIPs morphology and performance. Solid phase extraction and batch/continuous sorption processes were used to evaluate the polyphenols uptake/release in individual/competitive assays. Among the prepared MIPs, a suspension polymerization synthesized material, with 4-vinylpyridine as the functional monomer and water/methanol as solvent, showed a superior performance. The underlying cause of such a significant outcome is the likely surface imprinting process caused by the amphiphilic properties of polydatin. The uptake and subsequent selective release of polyphenols present in natural extracts was successfully demonstrated, considering a red wine solution as a case study. However, hydrophilic/hydrophobic interactions are inevitable (especially with complex natural extracts) and the tuning of the polarity of the solvents is an important issue for the isolation of the different polyphenols.