Analysis of human HPRT deletion mutations with X-linked probes and pulsed field gel electrophoresis.
Analysis of human HPRT deletion mutations with X-linked probes and pulsed field gel electrophoresis.
复制标题
使用 X 连锁探针和脉冲场凝胶电泳分析人类 HPRT 缺失突变。
DOI：
10.1002/em.2850180412
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发表时间：
1991
影响因子：
2.8
通讯作者：
Albertini,RJ
中科院分区：
文献类型：
--
作者：
Nicklas,JA;Lippert,MJ;Hunter,TC;O'Neill,JP;Albertini,RJ
由于人类 hprt 基因被用于许多突变研究，因此充分表征该基因非常重要。因此，我们实验室着手绘制人类X染色体q26带hprt基因周围区域的图谱。利用通过hprt克隆测定分离的hprt突变T细胞克隆（其删除了全部或部分hprt基因），我们订购了5个先前已知在Xq26中定位的匿名探针。结果表明，该区域包含 460 kb 至 18 Mb 的 DNA，至少是hprt 基因本身 (43 kb) 大小的 10 倍。对该区域的脉冲场凝胶分析正在进行中，以确定每个匿名探针和 hpr 之间的确切距离，并确定突变 T 细胞克隆中的缺失大小。
Because thehuman hprtgene is used in numerous mutation studies, it is important to fully characterize this gene. Therefore, our laboratory has undertaken to map the region around thehprtgene at band q26 of the human X chromosome. Utilizinghprtmutant T‐cell clones isolated using thehprtclonal assay, which have deletions of all or part of thehprtgene, we have ordered 5 anonymous probes previously known to map in Xq26. Results suggest that this region includes between 460 kb and 18 Mb of DNA, which is at least 10 times the size of thehprtgene itself (43 kb). Pulsed field gel analysis of the region is underway to determine the exact distances between each of the anonymous probes andhprtand to determine deletion sizes in the mutant T‐cell clones.