Two microPeptides are translated from a KSHV polycistronic RNA in human cells by leaky scanning mechanism.
Two microPeptides are translated from a KSHV polycistronic RNA in human cells by leaky scanning mechanism.
复制标题
两种微肽是通过漏扫描机制从人体细胞中的 KSHV 多顺反子 RNA 翻译而来的。
DOI：
10.1016/j.bbrc.2019.11.087
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发表时间：
2020
影响因子：
3.1
通讯作者：
Yuan,Yan
中科院分区：
文献类型：
--
作者：
Xu,Lei;Yuan,Yan
卡波西肉瘤相关疱疹病毒 (KSHV) 在开放阅读框 50 (RTA) 基因的相反链中编码 3.0 kb 多腺苷酸化 RNA (T3.0)。 T3.0 被错误注释为非编码 RNA，但发现它与核糖体相关，并且携带至少四个可翻译的 sORF。其中两个，即 vSP-1 和 vSP-2，已得到表征。 vSP-1 通过阻断 RTA 自身泛素化和蛋白酶体相关降解来增强 RTA 表达。 T3.0 RNA是多顺反子RNA。此外，在大多数从真核生物中先前注释的非编码RNA翻译的小肽（微肽）的情况下都观察到多顺反子翻译。为了阐明真核细胞中多顺反子 sORF 翻译的机制，我们发现 T3.0 RNA 通过渗漏扫描机制翻译 vSP-1 和 vSP-2。
Kaposi’s sarcoma-associated herpesvirus (KSHV) encodes a 3.0 kb polyadenylated RNA (T3.0) in the opposite strand of the open reading frame 50 (RTA) gene. The T3.0 was mis-annotated as a noncoding RNA but found to be associated with ribosomes and carries at least four translatable sORFs. Two of them, namely vSP-1 and vSP-2, have been characterized. vSP-1 enhances RTA expression by blocking RTA self-ubiquitylation and proteasome-associated degradation. T3.0 RNA is a polycistronic RNA. Furthermore, polycistronic translation has been observed in most of the cases of small peptides (microPeptides) translated from previously annotated noncoding RNAs in eukaryotes. In an effort to elucidate the mechanism underlying polycistronic sORF translation in eukaryotic cells, we found that T3.0 RNA translates vSP-1 and vSP-2 through a leaky scanning mechanism.