Regulation of Electromagnetic Perceptive Gene Using Ferromagnetic Particles for the External Control of Calcium Ion Transport
Regulation of Electromagnetic Perceptive Gene Using Ferromagnetic Particles for the External Control of Calcium Ion Transport
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DOI：
10.3390/biom10020308
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发表时间：
2020-02-01
期刊：
影响因子：
5.5
通讯作者：
Choi, Jonghoon
中科院分区：
文献类型：
--
作者：
Hwang, Jangsun;Choi, Yonghyun;Choi, Jonghoon
开发合成生物设备，允许在体内非侵入性地控制细胞的命运和功能，可能会彻底改变再生医学领域。为了解决这一未满足的需求，我们设计了一种人工生物“开关”，它由两部分组成：（1）电磁感知基因（EPG）和（2）磁性粒子。我们的小组最近从Kryptopterus bichtophis（玻璃鲶鱼）中克隆了EPG。EPG基因编码一种推定的膜相关蛋白，对电磁场（EMF）作出反应。该基因的主要作用机制是通过EMF刺激提高细胞内钙水平或改变流量。在这里，我们开发了一种用于远程调节[Ca 2 +]（i）的系统（即，细胞内钙离子浓度）。结果表明，EPG-FMPs可作为分子钙开关表达目的蛋白。该技术具有控制基因表达、药物递送和药物开发的潜力。
Developing synthetic biological devices to allow the noninvasive control of cell fate and function, in vivo can potentially revolutionize the field of regenerative medicine. To address this unmet need, we designed an artificial biological "switch" that consists of two parts: (1) the electromagnetic perceptive gene (EPG) and (2) magnetic particles. Our group has recently cloned the EPG from the Kryptopterus bicirrhis (glass catfish). The EPG gene encodes a putative membrane-associated protein that responds to electromagnetic fields (EMFs). This gene's primary mechanism of action is to raise the intracellular calcium levels or change in flux through EMF stimulation. Here, we developed a system for the remote regulation of [Ca2+](i) (i.e., intracellular calcium ion concentration) using streptavidin-coated ferromagnetic particles (FMPs) under a magnetic field. The results demonstrated that the EPG-FMPs can be used as a molecular calcium switch to express target proteins. This technology has the potential for controlled gene expression, drug delivery, and drug developments.