Endogenous glycosphingolipid acceptor specificity of sialosyltransferase systems in intact Golgi membranes, synaptosomes, and synaptic plasma membranes from rat brain.
Endogenous glycosphingolipid acceptor specificity of sialosyltransferase systems in intact Golgi membranes, synaptosomes, and synaptic plasma membranes from rat brain.
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大鼠脑完整高尔基体膜、突触体和突触质膜中唾液酸基转移酶系统的内源糖鞘脂受体特异性。
DOI：
10.1021/bi00410a036
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Rosenberg,A
中科院分区：
文献类型：
--
作者：
Durrie,R;Saito,M;Rosenberg,A
材料和方法试剂具有可从生化供应商获得的最高纯度，并且用在玻璃装置中再蒸馏的蒸馏去离子水制备溶液。脑亚组分制备中的所有均质化和离心步骤均在0-4 ℃下进行.大鼠脑的亚分级。断头后，将28日龄Sprague-Dawley大鼠幼鼠的前脑再次移动并置于10体积的冰冷的0.32 M蔗糖[1.0 mM三（羟甲基）氨基甲烷盐酸盐（Tris-HCl）-01 mM乙二胺四乙酸（EDTA），pH 7.2]中，然后匀浆并通过离心法分级。
Materials and MethodsReagents were of the highest purity available from biochemical suppliers, and solutions were prepared with distilled—deionized water redistilled in a glass apparatus. All homogenization and centrifugation steps in the preparation of the brain subfractions were carried out at 0-4 C. Subfractionation of Rat Brain. After decapitation, the forebrains of 28-day-old Sprague-Dawley ratpups were re-moved and placed in 10 volumes of ice-cold 0.32 M sucrose [1.0 mM tris (hydroxymethyl) aminomethane hydrochloride (Tris-HCl)-Ol mM ethylenediaminetetraacetic acid (EDTA), pH 7.2] and were then homogenized and fractionated by