Invasion of human oral epithelial cells by Prevotella intermedia
Invasion of human oral epithelial cells by Prevotella intermedia
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DOI：
10.1128/iai.66.12.6054-6057.1998
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发表时间：
1998-12-01
影响因子：
3.1
通讯作者：
Progulske-Fox, A
中科院分区：
文献类型：
--
作者：
Dorn, BR;Leung, KP;Progulske-Fox, A
致病性口腔细菌对口腔上皮细胞的侵袭可能是牙周病进展中的一个重要毒力因子。在这里，我们报告了中间普雷沃氏菌的临床分离株，菌株17，被发现侵入人口腔上皮细胞系（KB），而中间普雷沃氏菌27，另一个临床分离株，和中间普雷沃氏菌25611，模式菌株，没有被发现侵入细胞系。通过标准抗生素保护试验后活菌的回收来定量侵袭，并通过电子显微镜观察。细胞松弛素D、放线菌酮、单丹酰尸胺和低温（4 ° C）抑制中间青霉17的内化。抗中间疟原虫C型菌毛和抗全细胞抗体抑制侵袭，但抗C型菌毛抗体抑制侵袭的程度大于抗全细胞抗体。这项工作提供的证据表明，至少有一个菌株的中间疟原虫风扇侵入口腔上皮细胞系，C型菌毛和细胞骨架重排需要这种入侵。
Invasion of oral epithelial cells by pathogenic oral bacteria may represent an important virulence factor in the progression of periodontal disease. Here we report that a clinical isolate of Prevotella intermedia, strain 17, was found to invade a human oral epithelial cell line (KB), whereas P. intermedia 27, another clinical isolate, and P. intermedia 25611, the type strain, were not found to invade the cell line. Invasion was quantified by the recovery of viable bacteria following a standard antibiotic protection assay and observed by electron microscopy. Cytochalasin D, cycloheximide, monodansylcadaverine, and low temperature (4 degrees C) inhibited the internalization of P. intermedia 17. Antibodies raised against P. intermedia type C fimbriae and against whole cells inhibited invasion, but the anti-type-C-fimbria antibody inhibited invasion to a greater extent than the anti-whole-cell antibody. This work provides evidence that at least one strain of P. intermedia fan invade an oral epithelial cell line and that the type C fimbriae and a cytoskeletal rearrangement are required far this invasion.